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Myosin 4 Antibody (14-6503-82) in ICC/IF
Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 cells and C2C12 differentiated Myotubes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), eBioscience™ (Product # 14-6503-82) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbe... View More
产品信息
14-6503-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Chimpanzee,
Cat,
Guinea pig,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
已发表种属
C. elegans,
Chicken,
Human,
Mouse,
Rat,
Zebrafish
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
MF20
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.
The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.
Applications Reported: This MF20 antibody has been reported for use in western blotting, and immunocytochemistry.
Applications Tested: This MF20 antibody has been tested by immunocytochemistry and western blot and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
相关产品
免疫细胞化学
生物信息学
蛋白别名: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product
基因别名: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf
UniProt ID: (Human) Q9Y623, (Dog) Q076A5, (Rabbit) Q28641, (Mouse) Q5SX39, (Rat) Q29RW1
