Invitrogen

NEFL Monoclonal Antibody (T.400.5)

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

5个已发表图片

4 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

NEFL Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

NEFL Antibody (MA5-14981) in ICC/IF

Immunofluorescence analysis of NF-L was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-L Rabbit Monoclonal Antibody (Product # MA5-14981) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluo... View More

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NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated neurons, HEK-293 cell line; Mouse Cerebellum, Mouse Brain and Rat Brain tissue lysates in comparison to PC-12 and HeLa cell lines; Mouse Heart and Mouse Lung tissue lysates which are reported to be negative using Anti-NEFL Monoclonal Antibody (T.400.5) (Product # MA5-14981) in immunofluorescence analysis. {RE}

产品信息

MA5-14981

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

T.400.5

抗原

Synthetic peptide surrounding Glu450 of human Neurofilament-L

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

24 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10984147

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Scalable macroporous hydrogels enhance stem cell treatment of volumetric muscle loss.

Biomaterials

Eugenis I,Wu D,Hu C,Chiang G,Huang NF,Rando TA

MA5-14981 was used in Immunohistochemistry-immunofluorescence to investigate the scale-up potential of our scaffolds using a rat VML model and found that dECM-MA sponges significantly improved transplanted cell proliferation and muscle regeneration compared to conventional dECM scaffolds.

Tue Nov 01 00:00:00 EDT 2022

Targeted neural differentiation of murine mesenchymal stem cells by a protocol simulating the inflammatory site of neural injury.

Journal of tissue engineering and regenerative medicine

Chudickova M,Bruza P,Zajicova A,Trosan P,Svobodova L,Javorkova E,Kubinova S,Holan V

Published figure using NEFL monoclonal antibody (Product # MA5-14981) in Immunocytochemistry

Mon May 01 00:00:00 EDT 2017

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss.

Nature communications

Quarta M,Cromie M,Chacon R,Blonigan J,Garcia V,Akimenko I,Hamer M,Paine P,Stok M,Shrager JB,Rando TA

Published figure using NEFL monoclonal antibody (Product # MA5-14981) in Immunofluorescence

Tue Jun 20 00:00:00 EDT 2017

CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization.

PloS one

Nawaz MS,Giarda E,Bedogni F,La Montanara P,Ricciardi S,Ciceri D,Alberio T,Landsberger N,Rusconi L,Kilstrup-Nielsen C

MA5-14981 was used in western blot to characterize the interaction between Shootin1 and CDKL5 and how they work together to regulate neuronal polarization

Thu Jul 07 00:00:00 EDT 2016

生物信息学

蛋白别名: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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基因别名: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527

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Entrez Gene ID: (Human) 4747, (Mouse) 18039, (Rat) 83613

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功能

Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton protein binding protein C-terminus binding protein domain specific binding protein binding, bridging identical protein binding phospholipase binding protein heterodimerization activity structural molecule activity

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