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NFIL3 Antibody (PA5-106504) in ICC/IF
Immunocytochemistry analysis of NFIL3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NFIL3 polyclonal antibody (Product # PA5-106504) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-106504
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human NFIL3(Accession Q16649), corresponding to amino acid residues E429-G462.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854173
产品详细信息
Antibody detects endogenous levels of total NFIL3.
靶标信息
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E4 promoter binding-protein 4; E4 promoter-binding protein 4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF IL3A; nuclear factor interleukin 3 regulated protein; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; Transcriptional activator NF-IL3A
基因别名: AV225605; E4BP4; IL3BP1; NF-IL3A; NFIL3; NFIL3A
UniProt ID: (Human) Q16649, (Mouse) O08750, (Rat) Q6IMZ0
Entrez Gene ID: (Human) 4783, (Mouse) 18030, (Rat) 114519
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
sequence-specific DNA binding
basic leucine zipper transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
immune response
circadian rhythm
positive regulation of gene expression
cellular response to interleukin-4
transcription, DNA-templated
regulation of transcription, DNA-templated
rhythmic process
