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NG2 Antibody (MA5-55300) in WB
Western blot analysis of lysates from A375 cells, using NG2 Recombinant Monoclonal Antibody (Product # MA5-55300) at 1:1,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
产品信息
MA5-55300
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
6C9L7
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.05% ProClin 300
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Dry ice (international)
产品详细信息
Immunogen Sequence: LGAPLEGVLV ELEVLPAAIP LEAQNFSVPE GGSLTLAPPL LRVSGPYFPT LLGLSLQVLE PPQHGALQKE DGPQARTLSA FSWRMVEEQL IRYVHDGSET LTDSFVLMAN ASEMDRQSHP VAFTVTVLPV NDQPPILTTN TGLQMWEGAT APIPAEALRS TDGDSGSEDL VYTIEQPSNG RVVLRGAPGT EVRSFTQAQL DGGLVLFSHR GTLDGGFRFR LSDG
靶标信息
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; HMW MAA; MEL CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
基因别名: CSPG4; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
UniProt ID: (Human) Q6UVK1
Entrez Gene ID: (Human) 1464
activation of MAPK activity
angiogenesis
transmembrane receptor protein tyrosine kinase signaling pathway
cell proliferation
glial cell migration
cell migration
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
intracellular signal transduction
tissue remodeling
positive regulation of peptidyl-tyrosine phosphorylation
