OriGene

NIT2 Monoclonal Antibody (OTI3D5), TrueMAB™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他11个NIT2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (1)

NIT2 Antibody in Immunocytochemistry (ICC/IF)

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NIT2 Antibody (CF501097) in ICC/IF

Anti-NIT2 mouse monoclonal antibody (TA501097) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).

NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NIT2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in NIT2 knockout (KO) 293T cell line using Anti-NIT2 monoclonal antibody (Product # CF501097). {KO}

产品信息

CF501097

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:50

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:100

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

OTI3D5

抗原

Full length human recombinant protein of human NIT2 produced in HEK293T cell.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

仅用于科研。不用于诊断过程。未经明确授权不得转售。