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NKCC2 Antibody (PA5-88905) in WB
Western blot analysis of NKCC2 in extracts of various cell lines. Samples were incubated with NKCC2 Polyclonal antibody (Product # PA5-88905) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-88905
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_0003292)
偶联物
形式
Liquid
浓度
1.71 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2805209
产品详细信息
Immunogen sequence: FGDEAQKRLR ISFRPGNQEC YDNFLQSGET AKTDASFHAY DSHTNTYYLQ TFGHNTMDAV PKIEYYRNTG SISGPKVNRP SLLEIHEQLA KNVAVTPSSA D; Positive Samples: Mouse Kidney, Rat Kidney; Cellular Location: Membrane, Multi-pass membrane protein
靶标信息
The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BSC1; bumetanide-sensitive cotransporter type 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; MGC48843; mNKCC2; Na-K-2Cl cotransporter 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; Solute carrier family 12 member 1; Solute carrier family 12 member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter); Solute carrier family 12, member 1 (bumetanide-sensitive sodium-[potassium]-chloride cotransporter)
基因别名: AI788571; D630042G03Rik; mBSC1; Nkcc2; Slc12a1; urehr3
UniProt ID: (Mouse) P55014
Entrez Gene ID: (Mouse) 20495, (Rat) 25065
kidney development
transport
ion transport
potassium ion transport
sodium ion transport
chloride transport
excretion
protein insertion into membrane from inner side
ion transmembrane transport
renal rubidium ion absorption
chemical homeostasis
transmembrane transport
positive regulation of calcineurin-NFAT signaling cascade
cellular hyperosmotic salinity response
cation transmembrane transport
chloride transmembrane transport
diterpenoid metabolic process
renal sodium ion absorption
