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NLK Antibody (PA5-119756) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of NLK in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with NLK Polyclonal Antibody (Product # PA5-119756) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119756
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within C-terminal human NLK
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2913328
产品详细信息
Positive Control: SW480 cell lysate, HCT116 cell lysate, C2C12 cell lysate, MG-63, SW620, human uterus tissue, human small intestine tissue, human breast carcinoma tissue.
Subcellular Location: Nucleus, Cytoplasm.
靶标信息
NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Nemo-like kinase; nlk; Protein LAK1; serine/threonine protein kinase NLK; Serine/threonine-protein kinase NLK
基因别名: AI194375; LAK1; NLK
UniProt ID: (Human) Q9UBE8, (Mouse) O54949
Entrez Gene ID: (Human) 51701, (Mouse) 18099
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
transcription factor binding
ubiquitin protein ligase binding
SH2 domain binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
MAPK cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
transforming growth factor beta receptor signaling pathway
Wnt signaling pathway, calcium modulating pathway
peptidyl-threonine phosphorylation
negative regulation of Wnt signaling pathway
serine phosphorylation of STAT3 protein
intracellular signal transduction
protein autophosphorylation
protein stabilization
Wnt signaling pathway
phosphorylation
