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NME1 Antibody (CF801365) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME1 (RC220517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NME1. Positive lysates LY404982 (100 µg) and LC404982 (20 µg) can be purchased separately from OriGene.
产品信息
CF801365
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI4F11
抗原
Full length human recombinant protein of human NME1 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein
基因别名: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1
UniProt ID: (Human) P15531
Entrez Gene ID: (Human) 4830
magnesium ion binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein binding
ATP binding
GTP binding
intermediate filament binding
protein kinase binding
identical protein binding
gamma-tubulin binding
ribosomal small subunit binding
poly(A) RNA binding
kinase
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
DNA metabolic process
endocytosis
lactation
negative regulation of cell proliferation
nucleoside triphosphate biosynthetic process
negative regulation of gene expression
positive regulation of neuron projection development
response to amine
nucleobase-containing small molecule interconversion
hippocampus development
response to testosterone
cellular response to drug
regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
response to cAMP
cellular response to glucose stimulus
cellular response to fatty acid
