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NPM1 Antibody (A302-404A) in IHC
Detection of human and mouse NPM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- NPM1 (Cat. No. A302-404A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
产品信息
A302-404A
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 250 and 294 of human nucleophosmin.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
tris citrate/phosphate, pH 7-8
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine
靶标信息
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128
基因别名: B23; NO38; NPM; NPM1
UniProt ID: (Human) P06748, (Mouse) Q61937
Entrez Gene ID: (Human) 4869, (Mouse) 18148
transcription coactivator activity
RNA binding
protein kinase inhibitor activity
protein binding
protein kinase binding
Tat protein binding
histone binding
protein homodimerization activity
ribosomal large subunit binding
ribosomal small subunit binding
poly(A) RNA binding
protein heterodimerization activity
NF-kappaB binding
unfolded protein binding
nucleic acid binding
DNA binding
phosphatidylinositol-3,4,5-trisphosphate binding
rRNA binding
enzyme binding
protein N-terminus binding
chaperone
DNA repair
nucleosome assembly
intracellular protein transport
nucleocytoplasmic transport
response to stress
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
centrosome cycle
signal transduction
cell aging
protein localization
positive regulation of cell proliferation
negative regulation of cell proliferation
negative regulation of centrosome duplication
viral process
regulation of endodeoxyribonuclease activity
CENP-A containing nucleosome assembly
ribosome assembly
negative regulation of apoptotic process
negative regulation of protein kinase activity by regulation of protein phosphorylation
positive regulation of translation
positive regulation of transcription, DNA-templated
regulation of centriole replication
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
regulation of endoribonuclease activity
regulation of eIF2 alpha phosphorylation by dsRNA
positive regulation of cell cycle G2/M phase transition
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
rRNA export from nucleus
cell volume homeostasis
regulation of centrosome duplication
positive regulation of centrosome duplication
cell growth
positive regulation of cellular biosynthetic process
protein destabilization
ribosomal large subunit biogenesis
ribosomal small subunit biogenesis
positive regulation of catalytic activity
regulation of DNA damage response, signal transduction by p53 class mediator
regulation of neuron apoptotic process
positive regulation of DNA replication
positive regulation of protein kinase activity
negative regulation of mRNA splicing, via spliceosome
positive regulation of DNA metabolic process
protein homooligomerization
regulation of cell cycle
