Invitrogen

NQO1 Monoclonal Antibody (A180)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2个已发表图片

3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

NQO1 Antibody in Immunocytochemistry (ICC/IF)

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NQO1 Antibody (MA1-16672) in ICC/IF

Immunofluorescence analysis of NQO1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NQO1 Mouse Monoclonal Antibody (A180) (Product # MA1-16672) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a ... View More

NQO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NQO1 Antibody in Immunohistochemistry (IHC)

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NQO1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Abundant expression of NQO1 was observed in all the cell lines except SH-SY5Y and MOLT4 which showed little or no expression respectively using NQO1 Monoclonal Antibody (Product # MA1-16672) in Western Blot. {RE}

产品信息

MA1-16672

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100

免疫组化（冰冻） (IHC (F))

1:100

免疫细胞化学 (ICC/IF)

1:50

流式细胞分析 (Flow)

1-2 µg/mL

免疫沉淀 (IP)

1:1,000

产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A180

抗原

Full length recombinant NQO1 (from human lung).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2298735

产品详细信息

This antibody reacts weakly with mouse tissue.

靶标信息

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

E3 ligase TRIM15 facilitates non-small cell lung cancer progression through mediating Keap1-Nrf2 signaling pathway.

Cell communication and signaling : CCS

Liang M,Wang L,Sun Z,Chen X,Wang H,Qin L,Zhao W,Geng B

Published figure using NQO1 monoclonal antibody (Product # MA1-16672) in Western Blot

Mon May 09 00:00:00 EDT 2022

5,6-Dihydrocyclopenta[c][1,2]-dithiole-3(4H)-thione is a promising cancer chemopreventive agent in the urinary bladder.

Chemico-biological interactions

Paonessa JD,Munday CM,Mhawech-Fauceglia P,Munday R,Zhang Y

MA1-16672 was used in immunohistochemistry and western blot to elucidate the importance of Nrf2 in mediating the inductive activity of 5,6-dihydrocyclopenta[c][1,2]-dithiole-3 (4H)-thione

Mon Jun 15 00:00:00 EDT 2009

5,6-Dihydrocyclopenta[c][1,2]-dithiole-3(4H)-thione is a promising cancer chemopreventive agent in the urinary bladder.

Chemico-biological interactions

Paonessa JD,Munday CM,Mhawech-Fauceglia P,Munday R,Zhang Y

MA1-16672 was used in immunohistochemistry and western blot to elucidate the importance of Nrf2 in mediating the inductive activity of 5,6-dihydrocyclopenta[c][1,2]-dithiole-3 (4H)-thione

Mon Jun 15 00:00:00 EDT 2009

生物信息学

蛋白别名: Azoreductase; Diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase 4 (NADH/NADPH); diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase (quinone); NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H menadione oxidoreductase 1, dioxin inducible; NAD(P)H:menadione oxidoreductase; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1

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基因别名: AV001255; DHQU; DIA4; DTD; Nmo-1; Nmo1; NMOR1; NMORI; NQO1; Ox-1; Ox1; QR1

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UniProt ID: (Human) P15559, (Mouse) Q64669, (Rat) P05982

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Entrez Gene ID: (Dog) 610935, (Human) 1728, (Mouse) 18104, (Rat) 24314

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功能

NAD(P)H dehydrogenase (quinone) activity cytochrome-b5 reductase activity, acting on NAD(P)H superoxide dismutase activity protein binding identical protein binding poly(A) RNA binding oxidoreductase activity oxidoreductase

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