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NR0B2 Antibody (PA5-76632) in WB
Western blot analysis of NR0B2 in extracts of Mouse pancreas. Samples were incubated with NR0B2 polyclonal antibody (Product # PA5-76632) using a dilution of 1:500 followed by HRP Goat Anti-Rabbit IgG at 1:10,000 dilution. Lysates/proteins: 25 µg per Lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-76632
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length Human NR0B2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2720359
产品详细信息
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
靶标信息
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Nr0b2a; nuclear receptor SHP; Nuclear receptor subfamily 0 group B member 2; nuclear receptor subfamily 0, group B, member 2; Orphan nuclear receptor SHP; Small heterodimer partner; small heterodimer partner homologue
基因别名: NR0B2; SHP; SHP-1; SHP1
UniProt ID: (Human) Q15466, (Rat) P97947, (Mouse) Q62227
Entrez Gene ID: (Human) 8431, (Rat) 117274, (Mouse) 23957
DNA binding
steroid hormone receptor activity
transcription corepressor activity
protein binding
transcription factor binding
protein domain specific binding
protein complex binding
protein homodimerization activity
peroxisome proliferator activated receptor binding
retinoid X receptor binding
thyroid hormone receptor binding
transcription factor activity, sequence-specific DNA binding
retinoic acid receptor binding
C4 zinc finger nuclear receptor
DNA-binding transcription factor
zinc finger transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
Notch signaling pathway
cholesterol metabolic process
response to glucose
positive regulation of gene expression
negative regulation of gene expression
organ regeneration
positive regulation of insulin secretion
steroid hormone mediated signaling pathway
negative regulation of sequence-specific DNA binding transcription factor activity
transcription, DNA-templated
negative regulation of transcription, DNA-templated
regulation of transcription, DNA-templated
