Invitrogen

NUMB Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他23个NUMB抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

图: 1 / 5

NUMB Antibody (PA1-16831) in WB

Western blot was performed using Anti-NUMB Polyclonal Antibody (Product # PA1-16831) on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), PC-3 (Lane 2), DU 145 (Lane 3), A549 (Lane 4), SH-SY5Y (Lane 5), HeLa (Lane 6) and Mouse lung (Lane 7) and numb major isoform 65, 72 kDa was observed across cell lines except A549. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected... View More

Product Image Gallery

NUMB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with NUMB siRNA and reduction of signal was observed in Western Blot using NUMB Polyclonal Antibody (Product # PA1-16831). {KD}

产品信息

PA1-16831

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-0.5 µg/mL

免疫细胞化学 (ICC/IF)

1:500

产品规格

种属反应

Chicken, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide made to a C-terminal region of the murine numb protein sequence (between residues 600-653).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.5 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_568660

产品详细信息

The target sequence has 90% sequence homology with rat.

靶标信息

Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。