Bethyl Laboratories

Nanog Polyclonal Antibody

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Nanog Antibody (A300-397A) in WB

Detection of mouse Nanog by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody A300-397A (lot A300-397A-3) used for IP at 6 µg per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody A300-398A. For blotting immunoprecipitated Nanog, A300-397A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds... View More

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产品信息

A300-397A

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫沉淀 (IP)

2-5 µg/mg lysate

产品规格

种属反应

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Region between residues 1 and 50 of mouse Nanog homeobox.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

tris citrate/phosphate, pH 7-8

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

The recommended shelf life for this product is 1 year from date of receipt.

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ES cell-associated protein 4; FLJ12581; HGNC:20857; Homeobox protein NANOG; Homeobox transcription factor Nanog

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基因别名: 2410002E02Rik; Ecat4; ENK; Nanog

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UniProt ID: (Mouse) Q80Z64

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Entrez Gene ID: (Mouse) 71950

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功能

enhancer sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding transcription regulatory region DNA binding homeodomain transcription factor

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