Invitrogen

Nanog Monoclonal Antibody (23D2-3C6)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

11 篇参考文献

查看其他35个Nanog抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (1)

Nanog Antibody in Immunocytochemistry (ICC/IF)

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Nanog Antibody (MA1-017) in ICC/IF

Immunofluorescent analysis of Nanog (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody (Product # MA1-017) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by ... View More

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Nanog Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Western Blot analysis using Anti-Nanog Monoclonal Antibody (23D2-3C6) (Product # MA1-017) shows increased expression of Nanog in NTERA-2, NCCIT in comparison to HeLa, also the expression is decreased in iPSC upon differentiation to definitive endoderm. {RE}

产品信息

MA1-017

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50 -1:100

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

23D2-3C6

抗原

Full-length human recombinant protein expressed in bacteria

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536677

产品详细信息

Western blot analysis of MA1-017 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. MA1-017 shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase.

Journal of virology

Kettunen P,Lesnikova A,Räsänen N,Ojha R,Palmunen L,Laakso M,Lehtonen Š,Kuusisto J,Pietiläinen O,Saber SH,Joensuu M,Vapalahti OP,Koistinaho J,Rolova T,Balistreri G

MA1-017 was used in Immunocytochemistry-immunofluorescence to investigate the susceptibility of human induced pluripotent stem cell-derived neurons to SARS-CoV-2 infection, revealing a small fraction of neurons to be naturally susceptible and suggesting a potential mechanism involving late endosomal and lysosomal pathways for viral entry into these neurons.

Thu Apr 27 00:00:00 EDT 2023

Buprenorphine Exposure Alters the Development and Migration of Interneurons in the Cortex.

Frontiers in molecular neuroscience

Nieto-Estévez V,Donegan JJ,McMahon CL,Elam HB,Chavera TA,Varma P,Berg KA,Lodge DJ,Hsieh J

MA1-017 was used in Immunohistochemistry to suggest that more research is needed into the long-lasting consequences of buprenorphine exposure on the developing human brain.

Sat Oct 21 00:00:00 EDT 2023

TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling.

Cell reports

Sivakumar S,Qi S,Cheng N,Sathe AA,Kanchwala M,Kumar A,Evers BM,Xing C,Yu H

MA1-017 was used in Immunocytochemistry-immunofluorescence to probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs).

Tue Feb 15 00:00:00 EST 2022

TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling.

Cell reports

Sivakumar S,Qi S,Cheng N,Sathe AA,Kanchwala M,Kumar A,Evers BM,Xing C,Yu H

MA1-017 was used in Immunocytochemistry-immunofluorescence to probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs).

Tue Feb 15 00:00:00 EST 2022

Generation of four induced pluripotent stem cell lines (FHUi003-A, FHUi003-B, FHUi004-A and FHUi004-B) from two affected individuals of a familial neurohypophyseal diabetes insipidus family.

Stem cell research

Yoshida S,Okura H,Suga H,Soen M,Kawaguchi Y,Kurimoto J,Miyata T,Takagi H,Arima H,Fujikawa T,Otsuka F,Matsuyama A

MA1-017 was used in Immunocytochemistry, Flow Cytometry to produce disease-specific induced pluripotent stem cell lines that can aid disease recapitulation in vitro.

Thu Oct 01 00:00:00 EDT 2020

Generation of four induced pluripotent stem cell lines (FHUi003-A, FHUi003-B, FHUi004-A and FHUi004-B) from two affected individuals of a familial neurohypophyseal diabetes insipidus family.

Stem cell research

Yoshida S,Okura H,Suga H,Soen M,Kawaguchi Y,Kurimoto J,Miyata T,Takagi H,Arima H,Fujikawa T,Otsuka F,Matsuyama A

MA1-017 was used in Immunocytochemistry, Flow Cytometry to produce disease-specific induced pluripotent stem cell lines that can aid disease recapitulation in vitro.

Thu Oct 01 00:00:00 EDT 2020

Fluid shear stress induces cancer stem cell-like phenotype in MCF7 breast cancer cell line without inducing epithelial to mesenchymal transition.

International journal of oncology

Triantafillu UL,Park S,Klaassen NL,Raddatz AD,Kim Y

MA1-017 was used in Western Blotting to suggest that CTCs can be modeled in vitro as non-adherent cancer cells in FSS and that they show an increased CSC-like signature during circulation, providing new insights to the importance of CSC-targeting strategies when treating metastatic patients.

Wed Mar 01 00:00:00 EST 2017

Generation of an iPSC line from a patient with GTP cyclohydrolase 1 (GCH1) deficiency: HDMC0061i-GCH1.

Stem cell research

Jung-Klawitter S,Ebersold J,Göhring G,Blau N,Opladen T

MA1017 was used in immunocytochemistry to report the first model system to investigate the pathomechanism underlying GTP cyclohydrolase 1 mutations

Sat Apr 01 00:00:00 EDT 2017

Conservative site-specific and single-copy transgenesis in human LINE-1 elements.

Nucleic acids research

Vijaya Chandra SH,Makhija H,Peter S,Myint Wai CM,Li J,Zhu J,Ren Z,D'Alcontres MS,Siau JW,Chee S,Ghadessy FJ,Dröge P

MA1-017 was used in western blot to discuss Phage lambda integrase as a transgenesis tool

Thu Apr 07 00:00:00 EDT 2016

Generation of an iPSC line from a patient with tyrosine hydroxylase (TH) deficiency: TH-1 iPSC.

Stem cell research

Jung-Klawitter S,Blau N,Sebe A,Ebersold J,Göhring G,Opladen T

MA1017 was used in immunocytochemistry to generate and characterize tyrosine hydroxylase-expressing iPSCs

Tue Nov 01 00:00:00 EDT 2016

Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation.

Stem cell reports

Balboa D,Weltner J,Eurola S,Trokovic R,Wartiovaara K,Otonkoski T

MA1-017 was used in immunocytochemistry to demonstrate that dCas9 activator controls human pluripotent stem cell differentiation into endodermal lineages