Invitrogen

Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 550

Nanog Antibody in Immunocytochemistry (ICC/IF)

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Nanog Antibody (MA1-017-D550) in ICC/IF

Immunofluorescent analysis of Nanog (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated Nanog monoclonal antibody (Product # MA1-017-D550) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with... View More

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产品信息

MA1-017-D550

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:10-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

23D2-3C6

抗原

Full-length human recombinant protein expressed in bacteria

查看免疫原

偶联物

DyLight™ 550 DyLight™ 550 DyLight™ 550

激发/发射光谱

556/570 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with proprietary stabilizer

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536679

产品详细信息

MA1-017-D550 has been successfully used in ICC/IF applications with human samples.

MA1-017-D550 can be used for immunofluoresence analysis of Nanog in human iPSCs.

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。