Invitrogen

Nestin Monoclonal Antibody (10C2), Alexa Fluor™ 488, eBioscience™

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (53-9843-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized SKNSH cells using 10 µg/mL of Mouse IgG1 kappa Isotype Control Alexa Fluor® 488 (Product # 53-4714-42) (left) or 10 µg/mL Anti-Human Nestin Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Nestin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Nestin Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

Nestin Antibody in Immunohistochemistry (IHC)

Nestin Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

53-9843-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

10C2

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1834346

产品详细信息

Description: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.

Applications Reported: This 10C2 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining, and flow cytometric analysis.

Applications Tested: This 10C2 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Tue May 04 00:00:00 EDT 2021

Pannexin 1 Influences Lineage Specification of Human iPSCs.

Frontiers in cell and developmental biology

Noort RJ,Christopher GA,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Tue May 04 00:00:00 EDT 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Wed Dec 22 00:00:00 EST 2021

Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.

Biomolecules

Christopher GA,Noort RJ,Esseltine JL

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunocytochemistry

Wed Dec 22 00:00:00 EST 2021

Initiation of Pancreatic Cancer: The Interplay of Hyperglycemia and Macrophages Promotes the Acquisition of Malignancy-Associated Properties in Pancreatic Ductal Epithelial Cells.

International journal of molecular sciences

Otto L,Rahn S,Daunke T,Walter F,Winter E,Möller JL,Rose-John S,Wesch D,Schäfer H,Sebens S

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Western Blot

Tue May 11 00:00:00 EDT 2021

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2020

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2020

Metalloproteinases ADAM10 and ADAM17 Mediate Migration and Differentiation in Glioblastoma Sphere-Forming Cells.

Molecular neurobiology

Siney EJ,Holden A,Casselden E,Bulstrode H,Thomas GJ,Willaime-Morawek S

53-9843 was used in Flow cytometry/Cell sorting to explore the role of the metalloproteinases, ADAM10 and ADAM17, on the adhesive and migratory properties of human glioma sphere-forming cells.

Sat Jul 01 00:00:00 EDT 2017

Sphere-Derived Multipotent Progenitor Cells Obtained From Human Oral Mucosa Are Enriched in Neural Crest Cells.

Stem cells translational medicine

Abe S,Yamaguchi S,Sato Y,Harada K

53-9843 was used in Immunohistochemistry-immunofluorescence to isolate neural crest stem cells from oral mucosa using the neurosphere technique and establish in vivo bone tissue regeneration methods.

Fri Jan 01 00:00:00 EST 2016

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Histone modification profiling reveals differential signatures associated with human embryonic stem cell self-renewal and differentiation.

Proteomics

Bhanu NV,Sidoli S,Garcia BA

Published figure using Nestin monoclonal antibody (Product # 53-9843-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Establishment and characterization of primary glioblastoma cell lines from fresh and frozen material: a detailed comparison.

PloS one

Mullins CS,Schneider B,Stockhammer F,Krohn M,Classen CF,Linnebacher M

53-9843 was used in Flow cytometry/Cell sorting to evaluate a very simple cryopreservation procedure for GBM tissue prior to model establishment that will considerably reduce the logistic complexity.