Invitrogen

Nestin Monoclonal Antibody (196908), PE

Nestin Antibody in Flow Cytometry (Flow)

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Nestin Antibody (MA5-23633) in Flow

Flow cytometry of Nestin in NTera‚2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 μM Retinoic Acid for 60 days (light orange filled histogram). Samples were incubated in Nestin monoclonal antibody (Product # MA5-23633) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.

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产品信息

MA5-23633

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

10 µL per million cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

196908

抗原

NS0 mouse myeloma cell line transfected with human Nestin

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

纯化类型

Protein A/G

保存液

Buffered saline solution with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2608685

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。