Invitrogen

Nestin Monoclonal Antibody (2Q178)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

Nestin Antibody in Immunocytochemistry (ICC/IF)

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Nestin Antibody (MA1-91657) in ICC/IF

Immunofluorescence analysis of Nestin was performed using 70% confluent log phase PC-12 cells differentiated to neurons. The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 10 Minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nestin Monoclonal Antibody (2Q178) (Product # MA1-91657) at 10 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa ... View More

Nestin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nestin Antibody in Immunohistochemistry (IHC)

Nestin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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产品信息

MA1-91657

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20-1:200

免疫组化（冰冻） (IHC (F))

1:20-1:200

免疫细胞化学 (ICC/IF)

1:20-1:200

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产品规格

种属反应

Mouse, Rat

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

2Q178

抗原

Full length native Nestin protein purified from embryonic rat spinal cord.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

TBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2151007

产品详细信息

For use in ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50mM sodium borate at p.H. 9.5. for IHC-P and IHC-Fr tissue can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.

The predicted molecular weight of MA1-91657 is ~200kDa.

靶标信息

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

仅用于科研。不用于诊断过程。未经明确授权不得转售。