Invitrogen

NeuN Recombinant Rabbit Monoclonal Antibody (14H6L24)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2个已发表图片

8 篇参考文献

查看其他30个NeuN抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

NeuN Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

NeuN Antibody (702022) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized SH-SY5Y cells for detection of endogenous Fox3 using Anti- Fox3 Recombinant Rabbit Monoclonal Antibody (Product # 702022, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Fox3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938... View More

NeuN Antibody in Immunoprecipitation (IP)

NeuN Antibody in RNA Immunoprecipitation (RIP)

NeuN Antibody in Immunohistochemistry (IHC)

Product Image Gallery

NeuN Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells owing to their inherent genetic constitution. Relative expression of NeuN was observed in Mouse and Rat Brain and Cerebellum and not Mouse and Rat Heart and Kidney tissues using NeuN Monoclonal Antibody (Product # 702022) in western blot. {RE}

产品信息

702022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

3 µg/mL

免疫沉淀 (IP)

Assay-dependent

RNA免疫共沉淀 (RIP)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Avian, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

14H6L24

抗原

Protein corresponding to Human RBFOX3 (aa 1-312)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2633050

产品详细信息

This antibody is predicted to react with Monkey, Rat and Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Fox-3, also known as NEUN, HRNBP3, RBFOX3 (RNA binding protein, fox-1) or Fox-1 homolog C, is a 350 amino acid protein that contains one RRM (RNA recognition motif) domain. Localized to both the nucleus and cytoplasm, Fox-3 is suggested to regulate alternative splicing events. Fox-3 is encoded by a gene located on human chromosome 17, which is comprised over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and LiFraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Aberrant crosstalk between insulin signaling and mTOR in young Down syndrome individuals revealed by neuronal-derived extracellular vesicles.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Perluigi M,Picca A,Montanari E,Calvani R,Marini F,Matassa R,Tramutola A,Villani A,Familiari G,Domenico FD,Butterfield DA,Oh KJ,Marzetti E,Valentini D,Barone E

Published figure using NeuN recombinant monoclonal antibody (Product # 702022) in Western Blot

Mon Aug 01 00:00:00 EDT 2022

Aberrant crosstalk between insulin signaling and mTOR in young Down syndrome individuals revealed by neuronal-derived extracellular vesicles.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Perluigi M,Picca A,Montanari E,Calvani R,Marini F,Matassa R,Tramutola A,Villani A,Familiari G,Domenico FD,Butterfield DA,Oh KJ,Marzetti E,Valentini D,Barone E

Published figure using NeuN recombinant monoclonal antibody (Product # 702022) in Western Blot

Mon Aug 01 00:00:00 EDT 2022

Molecular Markers of Mechanosensation in Glycinergic Neurons in the Avian Lumbosacral Spinal Cord.

eNeuro

Stanchak KE,Miller KE,Lumsden EW,Shikiar D,Davis C,Brunton BW,Perkel DJ

702022 was used in Immunohistochemistry to suggest the LSO may be a modification and elaboration of a preexisting mechanosensory spinal network in vertebrates.

Fri Sep 16 00:00:00 EDT 2022

Developmental Regulation of Homeostatic Plasticity in Mouse Primary Visual Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Wen W,Turrigiano GG

702022 was used in Immunohistochemistry to suggest that homeostatic forms of plasticity can be recruited in a modular manner according to the evolving needs of a developing neural circuit.

Wed Dec 01 00:00:00 EST 2021

Small molecules efficiently reprogram apical papilla stem cells into neuron-like cells.

Experimental and therapeutic medicine

Chen Q,Yuan C,Jiang S,Heng BC,Zou T,Shen Z,Wang P,Zhang C

702022 was used in Western Blot to study the early phase of small molecule-induced neurogenic differentiation of stem cells from the apical papilla.

Tue Jun 01 00:00:00 EDT 2021

Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.

BioMed research international

Na L,Wang S,Liu T,Zhang L

702022 was used in Immunohistochemistry-immunofluorescence to investigate the curative effects of HUC-MSCs and USW on spinal cord injury and the influence MK2/TTP on the inflammatory microenvironment and its effects on spinal cord injury.

Tue Jun 08 00:00:00 EDT 2021

Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway.

BioMed research international

Na L,Wang S,Liu T,Zhang L

Tue Jun 08 00:00:00 EDT 2021

Fgr contributes to hemorrhage-induced thalamic pain by activating NF-κB/ERK1/2 pathways.

JCI insight

Huang T,Fu G,Gao J,Zhang Y,Cai W,Wu S,Jia S,Xia S,Bachmann T,Bekker A,Tao YX

702022 was used in Immunohistochemistry-immunofluorescence to suggest that Fgr is a key player in thalamic pain and a potential target for the therapeutic management of this disorder.

Thu Oct 15 00:00:00 EDT 2020

Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.

Frontiers in molecular neuroscience

Pronin A,Pham D,An W,Dvoriantchikova G,Reshetnikova G,Qiao J,Kozhekbaeva Z,Reiser AE,Slepak VZ,Shestopalov VI

702022 was used in Immunohistochemistry to map and characterise inflammasome complexes and detect pyroptosis in the ocular hypertension-injured retina.

Wed Sep 30 00:00:00 EDT 2020

Gemfibrozil, a Lipid-Lowering Drug, Lowers Amyloid Plaque Pathology and Enhances Memory in a Mouse Model of Alzheimer's Disease via Peroxisome Proliferator-Activated Receptor α.

Journal of Alzheimer's disease reports

Chandra S,Pahan K

702022 was used in Immunohistochemistry to highlight a new therapeutic property of gemfibrozil and suggest that this drug may be repurposed for treatment of AD.

Sat May 18 00:00:00 EDT 2019

生物信息学

蛋白别名: FLJ56884; FLJ58356; fox 1 homolog (C. elegans) 3; Fox-1 homolog C; Fox-3; fox1 homolog C; FOX3NeuN; hexaribonucleotide binding protein 3; hexaribonucleotide binding protein 3 (HRNBP3); Hexaribonucleotide-binding protein 3; NeuN antigen; neuronal nuclear antigen A60; neuronal nuclei; Neuronal nuclei antigen; RFOX3; RNA binding protein; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog (C. elegans) 3; RNA binding protein, fox-1 homolog 3

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基因别名: D11Bwg0517e; FOX-3; FOX3; HRNBP3; NEUN; Neuna60; RBFOX3; RGD1560070

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UniProt ID: (Human) A6NFN3, (Mouse) Q8BIF2

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Entrez Gene ID: (Human) 146713, (Rat) 287847, (Mouse) 52897

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功能

nucleotide binding DNA binding mRNA binding RNA binding nucleic acid binding protein binding RNA metabolism protein RNA processing factor RNA splicing factor nucleic acid metabolism protein

参与通路

regulation of alternative mRNA splicing, via spliceosome mRNA processing RNA splicing regulation of RNA splicing