Invitrogen

Nicastrin Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他17个Nicastrin抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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Nicastrin Antibody (PA5-17735) in WB

Western blot was performed using Nicastrin Polyclonal Antibody (Product # PA5-17735) and a 130 kDa band corresponding to Nicastrin was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of T98G (Lane 1), RSC96 (Lane 2), U-87 MG (Lane 3), IMR32 (Lane 4), Neuro 2a (Lane 5), HeLa (Lane 6), Hep G2 (Lane 7), MCF7 (Lane 8), Mouse Brain (Lane 9), Mouse Heart (Lane 10) and Rat Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then t... View More

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产品信息

PA5-17735

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to the carboxy-terminus of human Nicastrin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

183 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10981453

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

NCSTN (Nicastrin) is a gene that encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。