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Invitrogen

Nkx6.1 Polyclonal Antibody

View all (9) Nkx6.1 antibodies
Datasheet
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Datasheet
Protocols
Tech Support

Product Details

PA5-120724

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NKX6-1 (NP_006159.2).
View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.85 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2914296

Product Specific Information

Positive test controls include: BxPC-3, Rat pancreas.

Immunogen sequence: MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS SSSSSSSSSP SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND

Target Information

NKX6.1 is a bi-functional transcription factor that has effects in both the central nervous system and in the pancreas. It has both expression and repression effects on genes, leading to the proper formation of motoneurons, oligodendrocytes, and insulin-secreting beta cells. It is known to interact with Pax6 and Pdx-1, probably at or near the 60 amino acid homeobox DNA-binding domain. Due to its obvious relationship to beta cells and CNS, NKX6.1 is becoming a more interesting target for therapies in diabetes and certain brain diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Drosophila NK transcription factor related, gene family 6, locus 1; Homeobox protein NK-6 homolog A; Homeobox protein Nkx-6.1; homeodomain transcription factor Nkx6.1; NK homeo box, family 6, member A; NK homeobox, family 6, A; NK6 transcription factor homolog A; NK6 transcription factor related locus 1; NK6 transcription factor related, locus 1

Gene Aliases: NKX6-1; NKX6.1; Nkx61; NKX6A

UniProt ID: (Human) P78426, (Mouse) Q99MA9, (Rat) O35762

Entrez Gene ID: (Human) 4825, (Mouse) 18096, (Rat) 65193

Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding homeodomain transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter neuron migration transcription from RNA polymerase II promoter smoothened signaling pathway organ morphogenesis regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of axon extension pancreas development positive regulation of insulin secretion response to nicotine response to drug type B pancreatic cell proliferation positive regulation of neuron differentiation negative regulation of glial cell differentiation positive regulation of glial cell differentiation positive regulation of transcription from RNA polymerase II promoter oligodendrocyte differentiation positive regulation of sequence-specific DNA binding transcription factor activity detection of glucose cellular response to cytokine stimulus cellular response to peptide hormone stimulus type B pancreatic cell maturation positive regulation of type B pancreatic cell development regulation of neuron migration regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development cell proliferation central nervous system neuron differentiation neurogenesis cell differentiation endocrine pancreas development type B pancreatic cell development
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