Invitrogen

Nogo-A Polyclonal Antibody

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Nogo-A Antibody in Immunocytochemistry (ICC/IF)

Nogo-A Antibody in Immunocytochemistry (ICC/IF)

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Nogo-A Antibody (36-6600) in ICC/IF

The Biochemical journal 2013 - Figure 5 Up-regulation of Nogo-A and Apg-1 under hypoxic stress ( A ) Hippocampal neurons were cultured for 4 days in vitro and exposed to chemical ischaemia for 15 min, fixed after 8 h, and stained for Apg-1 and Nogo-A. Single-cell semi-quantitative immunocytochemistry was performed by defining a primary object area in the Nogo-A channel (red line). Pixel intensity was measured within this primary object a... View More

Nogo-A Antibody in Immunocytochemistry (ICC/IF)

Nogo-A Antibody in Immunocytochemistry (ICC/IF)

Nogo-A Antibody in Immunocytochemistry (ICC/IF)

Nogo-A Antibody in Immunocytochemistry (ICC/IF)

Nogo-A Antibody in Western Blot (WB)

Nogo-A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Nogo-A Antibody in Immunocytochemistry (ICC/IF) — Figure 5 Up-regulation of Nogo-A and Apg-1 under hypoxic stress ( A ) Hippocampal neurons were cultured for 4 days in vitro and exposed to chemical ischaemia for 15 min, fixed after 8 h, and stained for Apg-1 and Nogo-A. Single-cell semi-quantitative immunocytochemistry was performed by defining a primary object area in the Nogo-A channel (red line). Pixel intensity was measured within this primary object area in both the Nogo-A and Apg-1 channels and related to the number of Nogo-A-positive cells with an intact nucleus to yield the pixel intensity per neuron. Scale bar, 10 mum. ( B ) Quantification of single-cell Apg-1 and Nogo-A immunofluorescence under control conditions and following chemical ischaemia. Four independent experiments with a total number of approximately 400 neurons were analysed (Apg-1 compared with control: ** P =0.008, n =4; Nogo-A compared with control: * P =0.012, n =4; one-sample t test with Holm correction for multiplicity). con, control.

Nogo-A Antibody in Immunocytochemistry (ICC/IF) — Figure 6 Down-regulation of Nogo-A and Apg-1 under oxidative stress ( A ) Hippocampal neurons were cultured for 4 days in vitro and exposed to H 2 O 2 for 15 h, fixed and stained for Nogo-A and Apg-1. Single-cell semi-quantitative immunocytochemistry was performed by defining a primary object area in the Nogo-A channel (red line). Pixel intensity was measured within this primary object area in both the Nogo-A and Apg-1 channels and related to the number of Nogo-A-positive cells with an intact nucleus to yield the pixel intensity per neuron. Scale bar, 10 mum. ( B ) Quantification of single-cell Nogo-A and Apg-1 immunofluorescence under control conditions and following H 2 O 2 treatment. Four independent experiments with a total number of approximately 400 neurons were analysed (Apg-1 compared with control: P =0.061, n =4; Nogo-A compared with control: * P =0.018, n =4; one-sample t test with Holm correction for multiplicity). con, control.

Nogo-A Antibody in Immunocytochemistry (ICC/IF) — Figure 4 PLA with Nogo-A and Apg-1 ( A ) PLA with overexpressed Nogo-A-EGFP and EBFP2-Apg-1 in CHO-K1 cells. PLA signals (red) are specific only in double-transfected cells. Single-transfected cells as well as Nogo-B-EGFP/EBFP2-Apg1 double-transfected cells serve as a specificity control. ( B ) Confocal images of a PLA with endogenously expressed Nogo-A and Apg-1 in mouse hippocampal neurons. Nogo +/- mice were mated and hippocampal neurons from Nogo +/+ and Nogo -/- embryos were cultured for 20 days before fixed and subjected to PLA. PLA signals (red) are specific only in Nogo +/+ neurons. Neurons were visualized by neurofilament staining (green). Scale bars, 10 mum.

Nogo-A Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Topology of the NiG domain in primary hippocampal neurons Primary hippocampal neurons were cultured for 20 days before being fixed and completely permeabilized with 0.1% Triton X-100, followed by incubation with the 11C7 antibody, directed against the NiG domain, and anti-Apg-1 or anti-BiP antibody, or only plasma membrane permeabilized with 0.00125% digitonin in the presence of the respective primary antibodies and fixed thereafter. After selective permeabilization with digitonin, 11C7 is still immunoreactive, whereas the lumenal ER protein BiP is not, indicating a cytoplasmic localization of NiG, along with Apg-1. Scale bar, 10 mum.

Nogo-A Antibody in Western Blot (WB) — Published figure using Nogo-A polyclonal antibody (Product # 36-6600) in Western Blot

Nogo-A Antibody in Immunoprecipitation (IP) — Figure 2 Nogo-A interacts selectively with Apg-1 under endogenous conditions ( A ) Co-immunoprecipitation experiments with endogenously expressed RTN4-A/Nogo-A, RTN1-A and Apg-1 using mouse brain lysate. ( B ) Co-immunoprecipitation experiments with endogenously expressed Nogo-A, Hsp105 (A), and Apg-2 (B) using mouse brain lysate. A portion (10%) of the brain lysate that was used for co-immunoprecipitations serves as an immunoblotting control. IP, immunoprecipitation; rb, rabbit; WB, Western blot.

产品信息

36-6600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫细胞化学 (ICC/IF)

-

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免疫沉淀 (IP)

Assay-dependent

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其他PubMed (Misc)

-

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产品规格

种属反应

Rat

已发表种属

Hamster, Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the internal region of rat Nogo-A (Reticulon 4-A, Foocen, Glut4 Vesicle 20kDa Protein), which differs from the human sequence by only one amino acid.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533273

靶标信息

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress.

The Biochemical journal

Kern F,Stanika RI,Sarg B,Offterdinger M,Hess D,Obermair GJ,Lindner H,Bandtlow CE,Hengst L,Schweigreiter R

36-6600 was used in immunocytochemistry to identify Apg-1 as a novel interactor of NiG/Nogo-A

Tue Oct 15 00:00:00 EDT 2013

Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress.

The Biochemical journal

Kern F,Stanika RI,Sarg B,Offterdinger M,Hess D,Obermair GJ,Lindner H,Bandtlow CE,Hengst L,Schweigreiter R

36-6600 was used in immunocytochemistry to identify Apg-1 as a novel interactor of NiG/Nogo-A

Tue Oct 15 00:00:00 EDT 2013

Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress.

The Biochemical journal

Kern F,Stanika RI,Sarg B,Offterdinger M,Hess D,Obermair GJ,Lindner H,Bandtlow CE,Hengst L,Schweigreiter R

36-6600 was used in immunocytochemistry to identify Apg-1 as a novel interactor of NiG/Nogo-A

Tue Oct 15 00:00:00 EDT 2013

Neural MMP-28 expression precedes myelination during development and peripheral nerve repair.

Developmental dynamics : an official publication of the American Association of Anatomists

Werner SR,Mescher AL,Neff AW,King MW,Chaturvedi S,Duffin KL,Harty MW,Smith RC

Published figure using Nogo-A polyclonal antibody (Product # 36-6600) in Western Blot

Mon Oct 01 00:00:00 EDT 2007

生物信息学

蛋白别名: Foocen; Glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; Neurite outgrowth inhibitor; NI-220/250; Nogo protein; Reticulon-4

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基因别名: NI-250; Nogo; Nogo-A; r; rat N; rat NogoA; Rtn4; Vp20

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UniProt ID: (Rat) Q9JK11

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Entrez Gene ID: (Rat) 83765

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protein binding protein complex binding poly(A) RNA binding

参与通路

angiogenesis nervous system development axonal fasciculation aging negative regulation of neuron projection development response to activity olfactory nerve development cerebral cortex radial glia guided migration negative regulation of cell growth regulation of cell migration negative regulation of axon extension positive regulation of dopamine secretion negative regulation of neuron differentiation positive regulation of glial cell differentiation oligodendrocyte differentiation negative regulation of axonogenesis regulation of sensory perception of pain regulation of nervous system development cardiac epithelial to mesenchymal transition negative regulation of neuron projection regeneration endoplasmic reticulum tubular network organization regulation of branching morphogenesis of a nerve