Invitrogen

Nogo-A/B Polyclonal Antibody

Nogo-A/B Antibody in Immunocytochemistry (ICC/IF)

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Nogo-A/B Antibody (PA1-41220) in ICC/IF

Immunofluorescence analysis of Reticulon-4 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nogo-A/B Polyclonal Antibody (Product # PA1-41220) at (1:100 dilution) in 0.1% BSA, incubated at 4-degree Celsius overnight, and labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More

Nogo-A/B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nogo-A/B Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

PA1-41220

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

酶联免疫吸附实验 (ELISA)

Assay-Dependent

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Sheep, Rabbit, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2254188

产品详细信息

Suggested positive control: human, mouse or rat brain.

靶标信息

Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects.

Nature communications

Lee SH,Hadipour-Lakmehsari S,Murthy HR,Gibb N,Miyake T,Teng ACT,Cosme J,Yu JC,Moon M,Lim S,Wong V,Liu P,Billia F,Fernandez-Gonzalez R,Stagljar I,Sharma P,Kislinger T,Scott IC,Gramolini AO

Published figure using Nogo-A/B polyclonal antibody (Product # PA1-41220) in Immunoprecipitation

Wed Feb 19 00:00:00 EST 2020

REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects.

Nature communications

Lee SH,Hadipour-Lakmehsari S,Murthy HR,Gibb N,Miyake T,Teng ACT,Cosme J,Yu JC,Moon M,Lim S,Wong V,Liu P,Billia F,Fernandez-Gonzalez R,Stagljar I,Sharma P,Kislinger T,Scott IC,Gramolini AO

Published figure using Nogo-A/B polyclonal antibody (Product # PA1-41220) in Immunoprecipitation

Wed Feb 19 00:00:00 EST 2020

生物信息学

蛋白别名: Foocen; Glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; Human NogoA; MGC116054; My043 protein; neurite growth inhibitor 220; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NI-220/250; Nogo protein; NSP; reticulon 5; Reticulon-4; Reticulon-5; RTN-x

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基因别名: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; KIAA0886; mKIAA0886; mKIAA4153; My043; Nbla00271; Nbla10545; NgA; NI-250; NI220/250; NOGO; NOGO-A; Nogo-B; Nogo-C; NOGOC; NSP; NSP-CL; r; rat N; rat NogoA; RTN-X; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; SP1507; Vp20

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UniProt ID: (Human) Q9NQC3, (Mouse) Q99P72, (Rat) Q9JK11