Invitrogen

OCT4 Recombinant Rabbit Monoclonal Antibody (3H8L1.12)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (2)

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (703927) in ICC/IF

Knockdown of Oct-4 was achieved by transfecting iPSC cells with specific siRNA (Silencer® select Product # S10871; s10873). Immunofluorescence analysis was performed on iPSC cells (untransfected, panel a-d), transfected with Oct-4 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-Oct4 Recombinant Rabbit Monoclonal Antibody (Product # 703927, 1:16000 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More

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OCT4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. iPSC cells were transfected with Oct-4 siRNA and decrease in signal intensity was observed in western blot application using Anti-Oct-4 Rabbit Recombinant Monoclonal Antibody (Product # 703927, 1:5000 dilution). {KD}

产品信息

703927

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫细胞化学 (ICC/IF)

1:16,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

3H8L1.12

抗原

Protein corresponding to Human POU5F1 (aa 1-360)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2827381

产品详细信息

This antibody is predicted to react with Mouse.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1

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UniProt ID: (Human) Q01860

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Entrez Gene ID: (Human) 5460

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功能

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding

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