Invitrogen

OCT4 Monoclonal Antibody (9B7), DyLight™ 488

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1个已发表图片

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (MA1-104-D488) in ICC/IF

Immunofluorescence analysis of OCT-4 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OCT-4 Mouse Monoclonal Antibody (Product # MA1-104-D488) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-ac... View More

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OCT4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using OCT-4 Monoclonal Antibody (Product # MA1-104-D488) showed expression of OCT-4 in NTERA-2 and not in HeLa which is reported to be negative model for OCT-4 expression. {RE}

产品信息

MA1-104-D488

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG

分类

Monoclonal

类型

Antibody

克隆号

9B7

抗原

Full length human recombinant protein of human POU5F1 produced in E.coli.

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偶联物

DyLight™ 488 DyLight™ 488 DyLight™ 488

激发/发射光谱

492/519 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with proprietary stabilizer

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2536809

产品详细信息

MA1-104-D488 detects Oct4/Pou5F1 in human iPS samples.

MA1-104-D488 has been successfully used in ICC/IF applications.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.

Journal of cellular physiology

Bonaventura G,Iemmolo R,D'Amico AG,La Cognata V,Costanzo E,Zappia M,D'Agata V,Conforti FL,Aronica E,Cavallaro S

Published figure using OCT4 monoclonal antibody (Product # MA1-104-D488) in Immunofluorescence

Sun Apr 01 00:00:00 EDT 2018

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1

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UniProt ID: (Human) Q01860

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Entrez Gene ID: (Human) 5460

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功能

transcription regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding ubiquitin protein ligase binding miRNA binding sequence-specific DNA binding poly(A) RNA binding

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