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OLIG2 Antibody (MA5-35533) in WB
Western blot analysis of OLIG2 in extracts of various cell lines. Samples were incubated with OLIG2 Monoclonal antibody (Product # MA5-35533) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
产品信息
MA5-35533
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC1032
抗原
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Olig2 (Q13516)
偶联物
Unconjugated
形式
Liquid
浓度
0.43 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849433
产品详细信息
Immunogen sequence: AATAHPAAAA HAAHHPAVHH PILPPAAAAA AAAAAAAAVS SASLPGSGLP SVGSIRPPHG LLKSPSAAAA APLGGGGGGS GASGGFQHWG GMPCPCSMCQ V
靶标信息
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Basic helix loop helix protein class B1 (bHLHB1); basic helix-loop-helix protein 19 (bHLHe19); olig-2; Oligo2; Oligo2 antibody; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; Protein kinase C-binding protein 2 (PRKCBP2)
基因别名: AI604895; Bhlhb1; bHLHe19; Olg-2; Olig2; Oligo2; RK17
UniProt ID: (Mouse) Q9EQW6
Entrez Gene ID: (Mouse) 50913, (Rat) 304103
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein homodimerization activity
protein dimerization activity
HMG box domain binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
nervous system development
spinal cord motor neuron differentiation
spinal cord oligodendrocyte cell differentiation
spinal cord oligodendrocyte cell fate specification
thalamus development
myelination
negative regulation of neuron differentiation
neuron fate commitment
oligodendrocyte differentiation
positive regulation of oligodendrocyte differentiation
