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OPA1 Antibody (PA5-79771) in ICC/IF
Immunocytochemistry analysis of OPA1 using anti-OPA1 antibody (Product # PA5-79771). OPA1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-OPA1 antibody (Product # PA5-79771) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visual... View More
产品信息
PA5-79771
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2746886
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
靶标信息
OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: dynamin-like 120 kDa protein, mitochondrial; Dynamin-like GTPase OPA1, mitochondrial; dynamin-like guanosine triphosphatase; Large GTP-binding protein; LargeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; opa1 {ECO:0000312|EMBL:AAI11072.1}; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; Optic atrophy protein 1; Optic atrophy protein 1 homolog; RN protein
基因别名: 1200011N24Rik; AI225888; AI847218; BERHS; KIAA0567; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; NPG; NTG; OPA1
UniProt ID: (Human) O60313, (Rat) Q2TA68, (Mouse) P58281
Entrez Gene ID: (Human) 4976, (Rat) 171116, (Mouse) 74143
mitochondrial genome maintenance
mitochondrial fission
apoptotic process
mitochondrion organization
inner mitochondrial membrane organization
visual perception
mitochondrial fusion
positive regulation of mitochondrial fusion
positive regulation of neuron maturation
axonal transport of mitochondrion
negative regulation of release of cytochrome c from mitochondria
cellular senescence
positive regulation of dendrite development
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
neural tube closure
aging
metabolic process
negative regulation of intrinsic apoptotic signaling pathway
response to stimulus
