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OTUB1 Antibody (PA5-120082) in WB
Western blot analysis of OTUB1 in extracts of various cell lines. Samples were incubated with OTUB1 Polyclonal antibody (Product # PA5-120082) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
产品信息
PA5-120082
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human OTUB1 (NP_060140.2).
偶联物
形式
Liquid
浓度
0.09 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2913654
产品详细信息
Positive test controls include: 293T, Mouse intestine, Mouse liver. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: ERLELSVLYK EYAEDDNIYQ QKIKDLHKKY SYIRKTRPDG NCFYRAFGFS HLEALLDDSK ELQRFKAVSA KSKEDLVSQG FTEFTIEDFH NTFMDLIEQV E
靶标信息
The product of this gene is a member of the OTU superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deubiquitinating enzyme OTUB1; FLJ20113; FLJ40710; hOTU1; MGC111158; MGC4584; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; otubain 1; Otubain-1; Ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; Ubiquitin-specific-processing protease OTUB1
基因别名: AI850305; HSPC263; OTB1; OTU1; OTUB1
UniProt ID: (Human) Q96FW1, (Mouse) Q7TQI3
Entrez Gene ID: (Human) 55611, (Mouse) 107260
adaptive immune response
DNA repair
cellular response to DNA damage stimulus
protein K48-linked deubiquitination
cellular response to interleukin-1
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
immune system process
proteolysis
protein deubiquitination
