Invitrogen

OTX2 Recombinant Rabbit Monoclonal Antibody (14H14L5)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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3 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

OTX2 Antibody in Immunocytochemistry (ICC/IF)

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OTX2 Antibody (701948) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized NTERA-2 cells for detection of Otx2 using Otx2 Recombinant Rabbit Monoclonal Antibody (Product # 701948, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Otx2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) repre... View More

OTX2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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OTX2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of OTX2 was observed in Mouse retina in comparison to Mouse Brain, Mouse Eye and Mouse Lung using Anti-OTX2 Recombinant Rabbit Monoclonal Antibody (14H14L5) (Product # 701948) in Western Blot. {RE}

产品信息

701948

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 -1 µg/mL

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

14H14L5

抗原

Peptides corresponding to Human OTX2 (aa 20-39, 276-289)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2608961

产品详细信息

This antibody is predicted to react with Rat, Mouse, Pig and Sheep.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

OTX2 is a 289 amino acid containing protein encoding a member of the bicoid sub-family of homeodomain-containing transcription factors. It has two known alternative splice variants with distinct isoforms though presence of other isoforms cannot be excluded. The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. The protein may bind to BCD target sequence (BTS): 5-'TCTAATCCC-3'. Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Controlling neural territory patterning from pluripotency using a systems developmental biology approach.

iScience

Sears KE,Gullapalli K,Trivedi D,Mihas A,Bukys MA,Jensen J

701948 was used in Immunocytochemistry to demonstrate the importance of combinatorial control during neural induction and challenge the use of generic neural induction strategies such as dual-SMAD inhibition, when seeking to specify particular lineages from pluripotency.