Invitrogen

OXPAT Monoclonal Antibody (OTI3F2)

OXPAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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OXPAT Antibody (MA5-27505) in IHC (P)

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a PLIN5 monoclonal antibody (Product # MA5-27505) at a dilution of 1:500.

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产品信息

MA5-27505

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:500

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI3F2

抗原

Human recombinant protein fragment corresponding to amino acids 1-463 of PLIN5 produced in E.coli

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 1% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2724918

靶标信息

Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.

仅用于科研。不用于诊断过程。未经明确授权不得转售。