Search Thermo Fisher Scientific
图: 1 / 3
AA Sequence of recombinant protein: AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRRHSPH QALRKGVRRT RPSRKGKRLM TRGQLPR.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Oncostatin M. Anti-Human Oncostatin M-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hOncostatin M by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Oncostatin M (500-P30) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hOncostatin M.
Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hOncostatin M is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P30BT-1MG will be provided as 2 x 500 µg
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi's sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi's sarcoma cells, but inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptors in hepatoma cells. OSM shares several structural and functional characteristics with LIF, IL-6, and CNTF.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: MGC20461; Oncostatin-M; OSM
基因别名: OSM
UniProt ID: (Human) P13725
Entrez Gene ID: (Human) 5008
分子生物学功能:
cytokine
interleukin superfamily
intercellular signal molecule