Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 4
PAK3 Antibody (PA5-29075) in WB
Western blot was performed using Anti-PAK3 Polyclonal Antibody (Product # PA5-29075) and a 60 kDa band corresponding to PAK3 was observed across the cell lines and tissues tested except Mouse and Rat Stomach. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), NTERA-2 cl.D1 (Lane 3), U-2 OS (Lane 4), NIH/3T3 (Lane 5), A549 (Lane 6), A-431 (Lane 7), tissue extracts of Mouse Brain (Lane 8), Rat Brain (Lane 9), Mouse Stomach (Lane 10) and Rat Stomach (Lane 11) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Pro... View More
产品信息
PA5-29075
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein fragment corresponding to a region within amino acids 253 and 544 of Human PAK3
偶联物
Unconjugated
形式
Liquid
浓度
0.74 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2546551
产品详细信息
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, NIH-3T3, JC, mouse brain, rat brain.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (99%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: 2.7.11.1; adriamycin resistance-associated; Beta-PAK; CDC42/RAC effector kinase PAK-B; CDKN1A; hPAK3; Oligophrenin-3; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p65-PAK; PAK beta; PAK-3; Serine/threonine-protein kinase PAK 3
基因别名: ARA; beta-PAK; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3; PAK3beta; Pak65alpha; Pak65beta; Pakb; Stk4
UniProt ID: (Human) O75914, (Mouse) Q61036, (Rat) Q62829
Entrez Gene ID: (Human) 5063, (Mouse) 18481, (Rat) 29433
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein binding
ATP binding
Rho GTPase binding
SH3 domain binding
metal ion binding
nucleotide binding
catalytic activity
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
GTPase binding
MAPK cascade
activation of MAPK activity
protein phosphorylation
Rho protein signal transduction
multicellular organism development
regulation of mitotic cell cycle
axonogenesis
metabolic process
regulation of neuron projection development
phosphorylation
dendrite development
cell migration
signal transduction by protein phosphorylation
actin cytoskeleton organization
regulation of actin filament polymerization
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
regulation of MAPK cascade
positive regulation of neuron apoptotic process
dendritic spine development
positive regulation of dendritic spine morphogenesis
cellular response to organic cyclic compound
positive regulation of DNA biosynthetic process
mitotic cell cycle
intracellular signal transduction
stimulatory C-type lectin receptor signaling pathway
T cell costimulation
ephrin receptor signaling pathway
synapse organization
T cell receptor signaling pathway
dendritic spine morphogenesis
