Invitrogen

PAX6 Monoclonal Antibody (AD2.38), eBioscience™

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2 篇参考文献
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实验方案
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说明书
实验方案
技术支持

产品信息

14-9914-82

应用
建议稀释比
已发表文章

免疫印迹 (WB)

Assay-Dependent
-

免疫细胞化学 (ICC/IF)

5 µg/mL
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产品规格

种属反应

Chicken, Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

AD2.38

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10669586

产品详细信息

Description: This AD2.38 monoclonal antibody reacts with human, mouse, and rat Pax6. This 47-kDa transcription factor is expressed during embryonic neurogenesis in the brain and central nervous system, as well as in neuroepithelial cells of the retina. Pax6 is involved in development of the eyes, nose, central nervous system, pancreas, and pituitary gland. Studies also demonstrate that Pax6 is crucial for alpha-cell differentiation and glucagon biosynthesis in the pancreas. Mutation of Pax6 results in eye disorders such as aniridia in humans and Small eye in mice.

Applications Reported: This AD2.38 antibody has been reported for use in immunoblotting (WB) and immunocytochemistry.

Applications Tested: This AD2.38 antibody has been tested by immunocytochemistry of fixed and permeabilized MV+ cells. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene 6; paired box homeotic gene-6; Paired box protein Pax-6; small eye; transcription factor

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基因别名: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; PAX-6; PAX6; Sey; WAGR

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UniProt ID: (Human) P26367, (Rat) P63016, (Mouse) P63015

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Entrez Gene ID: (Chicken) 395943, (Human) 5080, (Rat) 25509, (Mouse) 18508

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding
参与通路
negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development cell fate determination neural crest cell migration neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation epithelial cell development lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell differentiation pancreatic A cell development regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter smoothened signaling pathway axonogenesis axon guidance central nervous system development brain development salivary gland morphogenesis negative regulation of cell proliferation response to wounding regulation of asymmetric cell division dorsal/ventral axis specification anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression positive regulation of gene expression regulation of neuron projection development protein ubiquitination spinal cord development pallium development cerebellum development rhombomere morphogenesis olfactory bulb development oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment telencephalon regionalization pituitary gland development interkinetic nuclear migration signal transduction involved in regulation of gene expression cell differentiation keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation forebrain development hindbrain development lacrimal gland development cellular response to insulin stimulus protein localization to organelle cellular response to drug eye photoreceptor cell development glucose homeostasis positive regulation of cell fate specification camera-type eye development cellular response to fibroblast growth factor stimulus cell fate commitment response to ethanol regulation of neuron differentiation negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis astrocyte differentiation negative regulation of epithelial cell proliferation regulation of neurogenesis negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye neural precursor cell proliferation positive regulation of glucagon secretion cellular response to glucose stimulus cellular response to prostaglandin E stimulus insulin metabolic process negative regulation of neural precursor cell proliferation positive regulation of neuron migration multicellular organism development anatomical structure development eye development visual perception organ morphogenesis neuron fate commitment transcription, DNA-templated regulation of transcription, DNA-templated
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