Invitrogen

PBX1 Polyclonal Antibody

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP)

PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP)

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PBX1 Antibody (PA5-82100) in WB, ICC/IF, IHC (P), IP

Respiratory research 2021 - Fig. 4 Interaction candidates are coexpressed with TBX2 in the pulmonary mesenchyme and interact in HEK293 cells. a Co-immunofluorescence analysis of candidate interaction partners (red) and TBX2 (green) on frontal sections of the right lung of E14.5 Tbx2 cre/ + embryos. Antigens are color-coded and nuclei were counterstained with DAPI (blue). Insets or selected regions in overview images are magnified in r... View More

PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP)

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PBX1 Antibody in Western Blot, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation (WB, ICC/IF, IHC (P), IP) — Fig. 4 Interaction candidates are coexpressed with TBX2 in the pulmonary mesenchyme and interact in HEK293 cells. a Co-immunofluorescence analysis of candidate interaction partners (red) and TBX2 (green) on frontal sections of the right lung of E14.5 Tbx2 cre/ + embryos. Antigens are color-coded and nuclei were counterstained with DAPI (blue). Insets or selected regions in overview images are magnified in rows 2,4 and 6. b In situ proximity ligation assay of TBX2 and candidate interaction partners on 10 um frontal sections of E14.5 wildtype and Tbx2 cre/fl mutant lungs. Direct interaction is visualized by small red fluorescent dots. Larger more diffuse orange stains are due to auto-fluorescence of blood cells. Nuclei are counterstained with DAPI (blue). c Western blot analysis of co-immunoprecipitation experiments for verification of TBX2 interaction with candidate proteins on 10% SDS polyacrylamide gels. Detection was performed with an anti-TBX2 primary antibody and developed with chemoluminescence-IHC. Arrows indicate TBX2 bands. Lanes were loaded as follows: No antibody: IP without specific antibody resembling negative IP-control; 5% input: 5% of crude cell extract before precipitation; empty: no protein loaded; IP: co-immunoprecipitate with antibody for specific candidate. Expected molecular weight for TBX2.HA approx. 76.2 kDa

PBX1 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of PBX1 was observed specifically in HeLa cells and in SK-MEL-30 cells using anti-PBX1 Polyclonal Antibody (Product #PA5-82100) in western blot. The relative expression levels of PBX1 within each cell line is shown using RNA-Seq. {RE}

产品信息

PA5-82100

应用

建议稀释比

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免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化 (IHC)

1:200-1:500

-

免疫组化（石蜡） (IHC (P))

-

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免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human PBX1. Recombinant protein control fragment (Product #RP-101910).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Anitgen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2789261

产品详细信息

Immunogen sequence: PRLMHSHAGV GMAGHPGLSQ HLQDGAGGTE GEGGRKQDIG DILQQIMTIT DQSLDEAQAR KHALNCHRMK PALFNVLCEI KEKTVLSIRG AQEEEPTDPQ LMRLDNMLLA EGV

靶标信息

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1; 19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.

Respiratory research

Lüdtke TH,Wojahn I,Kleppa MJ,Schierstaedt J,Christoffels VM,Künzler P,Kispert A

PA5-82100 was used in Immunoprecipitation, Immunohistochemistry (Paraffin) to suggest that TBX2 interacts with homeobox and HMG-box transcription factors as well as with the NuRD chromatin remodeling complex to repress transcription of anti-proliferative genes in the pulmonary mesenchyme.

Wed Mar 17 00:00:00 EDT 2021

生物信息学

蛋白别名: DKFZp686B09108; Homeobox protein PBX1; Homeobox protein PRL; MGC126627; OTTHUMP00000032205; pre-B-cell leukemia homeobox 1; Pre-B-cell leukemia transcription factor 1

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基因别名: 2310056B04Rik; D230003C07Rik; Pbx-1; PBX1; PRL

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UniProt ID: (Human) P40424, (Mouse) P41778

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Entrez Gene ID: (Human) 5087, (Mouse) 18514, (Rat) 304947

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein heterodimerization activity transcription factor binding sequence-specific DNA binding molecular_function homeodomain transcription factor

参与通路

branching involved in ureteric bud morphogenesis transcription from RNA polymerase II promoter steroid biosynthetic process sex differentiation positive regulation of cell proliferation anterior/posterior pattern specification proximal/distal pattern formation positive regulation of G2/M transition of mitotic cell cycle regulation of ossification adrenal gland development embryonic limb morphogenesis somatic stem cell population maintenance embryonic hemopoiesis negative regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter spleen development thymus development embryonic skeletal system development urogenital system development transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development organ morphogenesis cell differentiation regulation of cell proliferation negative regulation of sequence-specific DNA binding transcription factor activity embryonic organ development biological_process