Invitrogen

PCNA Polyclonal Antibody

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7 篇参考文献
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说明书
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产品信息

PA1-38424

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1 µg/mL
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免疫组化 (IHC)

-
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免疫组化（石蜡） (IHC (P))

1:100
-

免疫细胞化学 (ICC/IF)

5 µg/mL
-
产品规格

种属反应

Human, Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant human PCNA protein.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.14 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.6, with 1% BSA

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2160549

产品详细信息

A suggested positive control is tonsil, lymph node or small intestine tissue.

靶标信息

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

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基因别名: ATLD2; PCNA

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UniProt ID: (Human) P12004, (Mouse) P17918

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Entrez Gene ID: (Human) 5111, (Mouse) 18538

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功能
purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding DNA polymerase processivity factor
参与通路
G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication negative regulation of transcription from RNA polymerase II promoter DNA replication regulation of DNA replication DNA repair base-excision repair, gap-filling cellular response to DNA damage stimulus regulation of catalytic activity cellular response to xenobiotic stimulus
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