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Invitrogen
PCNA Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 7
PCNA Antibody (PA1-38424) in ICC/IF
Immunofluorescence analysis of PCNA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PCNA Polyclonal Antibody (Product # PA1-38424) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More
产品信息
PA1-38424
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant human PCNA protein.
偶联物
形式
Liquid
浓度
0.14 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 1% BSA
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2160549
产品详细信息
A suggested positive control is tonsil, lymph node or small intestine tissue.
靶标信息
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
基因别名: ATLD2; PCNA
UniProt ID: (Human) P12004, (Mouse) P17918
Entrez Gene ID: (Human) 5111, (Mouse) 18538
purine-specific mismatch base pair DNA N-glycosylase activity
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
DNA polymerase binding
DNA binding
transcription factor binding
DNA polymerase processivity factor
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
DNA strand elongation involved in DNA replication
leading strand elongation
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
heart development
cell proliferation
protein sumoylation
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
nucleotide-excision repair, DNA incision
response to lipid
cellular response to UV
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
error-free translesion synthesis
mitotic telomere maintenance via semi-conservative replication
negative regulation of transcription from RNA polymerase II promoter
DNA replication
regulation of DNA replication
DNA repair
base-excision repair, gap-filling
cellular response to DNA damage stimulus
regulation of catalytic activity
cellular response to xenobiotic stimulus
