Invitrogen

PCNA Recombinant Rabbit Monoclonal Antibody (SAIC-27C-101)

PCNA Antibody in Immunocytochemistry (ICC/IF)

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PCNA Antibody (MA5-47908) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of PCNA in HeLa cells using PCNA Recombinant Monoclonal Antibody (Product # MA5-47908). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with PCNA Recombinant Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing nuclear staining. The nuclear DNA stain is DAPI (blue). Panels show from left-right, top-bottom PCNA Recombinant Monoclonal Antibody, DAPI, merged channels and an isotype control. The isotype control was staine... View More

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产品信息

MA5-47908

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

10 µg/mL

Mass Spectrometry (MS)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG, lambda

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SAIC-27C-101

抗原

Peptide "LVQGSILK" derived from PCNA1 (Proliferating cellular nuclear antigen 1) conjugated to KLH.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3074352

产品详细信息

Specificity: Recognises human PCNA.

Preparation: Peptide "LVQGSILK" derived from PCNA1 (Proliferating cellular nuclear antigen 1) conjugated to KLH. PCNA ins an auxiliary and regulatory protein of DNA polymerase delta involved in control of eukaryotic DNA replication.

靶标信息

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。