NeoBiotechnologies

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Monoclonal Antibody (PC5)

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PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody (5111-MSM3-P0) in IHC (P)

Formalin-fixed, paraffin-embedded human colon carcinoma stained with PCNA Mouse Monoclonal Antibody (PC5).

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Western Blot (WB)

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in SDS-PAGE (SDS-PAGE)

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PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human colon carcinoma stained with PCNA Mouse Monoclonal Antibody (PC5).

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Mouse Monoclonal Antibody (PC5).

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in Western Blot (WB) — Western blot analysis of HePG2, THP-1, A431, A549, SKBr3, HEK293 and U937 cell lines using PCNA Mouse Monoclonal Antibody (PC5).

PCNA (Proliferating Cell Nuclear Antigen) (G1- and S-phase Marker) Antibody in SDS-PAGE (SDS-PAGE) — SDS-PAGE analysis Purified PCNA Mouse Monoclonal Antibody (PC5). Confirmation of Purity and Integrity of Antibody.

产品信息

5111-MSM3-P0

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1-2 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat, Yeast

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

PC5

抗原

Rat PCNA/Protein A fusion protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4, with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C, do not freeze

运输条件

Wet ice

靶标信息

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

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基因别名: ATLD2; PCNA; Pcna/cyclin; PCNAR; POL30; YBR0811; YBR088C

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UniProt ID: (Human) P12004, (Baker's yeast) D6VQ89, (Rat) P04961, (Mouse) P17918

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Entrez Gene ID: (Human) 5111, (Baker's yeast) 852385, (Rat) 25737, (Mouse) 18538

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purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding DNA polymerase processivity factor

参与通路

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication mitotic cell cycle meiotic mismatch repair DNA replication lagging strand elongation regulation of DNA replication DNA repair nucleotide-excision repair postreplication repair cellular response to DNA damage stimulus mitotic sister chromatid cohesion chromatin silencing at silent mating-type cassette telomeric heterochromatin assembly establishment of mitotic sister chromatid cohesion maintenance of DNA trinucleotide repeats positive regulation of exodeoxyribonuclease activity positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands positive regulation of exonuclease activity negative regulation of transcription from RNA polymerase II promoter base-excision repair, gap-filling cellular response to xenobiotic stimulus regulation of catalytic activity