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AA Sequence of recombinant protein: MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PEDF. Anti-Human PEDF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human PEDF by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PEDF (500-P263) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PEDF.
Western Blot: To detect Human PEDF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human PEDF is 1.5-3.0 ng/lane,under either reducing or non-reducing conditions.
500-P263BT-1MG will be provided as 2 x 500 µg
PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors, such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature-related neurodegenerative diseases, such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; PEDF; PIG 35; Pigment epithelium-derived factor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; SERPINF 1; sserpin family F member 1; testis tissue sperm-binding protein Li 70n
基因别名: EPC-1; OI12; OI6; PEDF; PIG35; SERPINF1
UniProt ID: (Human) P36955
Entrez Gene ID: (Human) 5176