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PERK Antibody (MA5-51928) in WB
Western blot was performed using PERK Rabbit Recombinant Monoclonal Antibody (PSH0-81) (Product # MA5-51928) and 140 kDa band corresponding to PERK was observed across cell lines tested. Whole cell extracts (20 µg lysate) of MCF7 (Lane 1), HepG2 (Lane 2), HEK-293 (Lane 3), HeLa (Lane 4), A549 (Lane 5), U-87 MG (Lane 6), and whole cell extracts (16 µg lysate) of K-562 (Lane 7) were electrophoresed using 4-20% SDS-PAGE gel. Resolved proteins were transferred onto a PVDF membrane. The blot was blocked with 5% NFDM/TBST for 1 hour at room temp... View More
产品信息
MA5-51928
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH0-81
抗原
Recombinant protein within human perk aa 51-450/1,116.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3093066
产品详细信息
Sequence Similarities: 87% Mouse/98%Rat.
Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.
Positive Control: MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate.
Subcellular Location: Endoplasmic reticulum membrane.
Predicted band size: 125 kDa.
靶标信息
EIF2AK3 (PERK) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to a decrease in protein sythesis. In the case of EIF2AK3, signaling is initiated by misfolded proteins in the ER. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS), also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2.7.11.1; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; OTTHUMP00000207187; OTTHUMP00000207188; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase
基因别名: EIF2AK3; PEK; PERK; WRS
UniProt ID: (Human) Q9NZJ5
Entrez Gene ID: (Human) 9451
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
protein binding
ATP binding
enzyme binding
protein phosphatase binding
identical protein binding
protein homodimerization activity
Hsp90 protein binding
non-receptor serine/threonine protein kinase
skeletal system development
ossification
angiogenesis
chondrocyte development
protein phosphorylation
activation of cysteine-type endopeptidase activity involved in apoptotic process
ER overload response
endoplasmic reticulum organization
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
regulation of translational initiation by eIF2 alpha phosphorylation
negative regulation of translation
peptidyl-serine phosphorylation
calcium-mediated signaling
insulin secretion
bone mineralization
endoplasmic reticulum unfolded protein response
endocrine pancreas development
negative regulation of myelination
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
response to endoplasmic reticulum stress
eiF2alpha phosphorylation in response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
cellular response to glucose starvation
positive regulation of transcription from RNA polymerase I promoter
protein autophosphorylation
insulin-like growth factor receptor signaling pathway
protein homooligomerization
regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation
cellular response to cold
positive regulation of protein localization to nucleus
regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
response to manganese-induced endoplasmic reticulum stress
