Invitrogen

PPARGC1A Polyclonal Antibody

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PPARGC1A Antibody (PA5-72948) in WB

Western blot was performed using Anti-PGC1 alpha Polyclonal Antibody(Product # PA5-72948) and a 91kDa band corresponding to PGC1 alpha was observed across the tested cell lines. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), NIH/3T3 (Lane 4), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the prima... View More

PPARGC1A Antibody in Immunoprecipitation (IP)

PPARGC1A Antibody in RNA Immunoprecipitation (RIP)

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产品信息

PA5-72948

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

1:1,000

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免疫沉淀 (IP)

Assay-Dependent

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RNA免疫共沉淀 (RIP)

Assay-dependent

产品规格

种属反应

Hamster, Human, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2718802

靶标信息

PGC1 alpha is a transcriptional coactivator that regulates the genes involved in energy metabolism. It interacts with PPARgamma, which permits it's interaction with multiple transcription factors. PGC-1 Alpha can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. It may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Rescue of Mitochondrial Function in Hutchinson-Gilford Progeria Syndrome by the Pharmacological Modulation of Exportin CRM1.

Cells

Monterrubio-Ledezma F,Navarro-García F,Massieu L,Mondragón-Flores R,Soto-Ponce LA,Magaña JJ,Cisneros B

PA5-72948 was used in Immunocytochemistry to analyze the effect of LMB on mitochondrial function.

Tue Jan 10 00:00:00 EST 2023

Rescue of Mitochondrial Function in Hutchinson-Gilford Progeria Syndrome by the Pharmacological Modulation of Exportin CRM1.

Cells

Monterrubio-Ledezma F,Navarro-García F,Massieu L,Mondragón-Flores R,Soto-Ponce LA,Magaña JJ,Cisneros B

PA5-72948 was used in Immunocytochemistry to analyze the effect of LMB on mitochondrial function.

Tue Jan 10 00:00:00 EST 2023

Altered expression, but small contribution, of the histone demethylase KDM6A in obstructive uropathy in mice.

Disease models & mechanisms

Hong LYQ,Yeung ESH,Tran DT,Yerra VG,Kaur H,Kabir MDG,Advani SL,Liu Y,Batchu SN,Advani A

PA5-72948 was used in Western Blotting to demonstrate Kdm6a to be a dynamically regulated gene in the kidney tubule, varying in expression levels by sex and in response to injury.

Fri Sep 01 00:00:00 EDT 2023

AMPK Activation Is Indispensable for the Protective Effects of Caloric Restriction on Left Ventricular Function in Postinfarct Myocardium.

Biology

Niemann B,Pan R,Issa H,Simm A,Schulz R,Rohrbach S

Published figure using PGC1 alpha polyclonal antibody (Product # PA5-72948) in Western Blot

Wed Mar 16 00:00:00 EDT 2022

Impact of 4 weeks of western diet and aerobic exercise training on whole-body phenotype and skeletal muscle mitochondrial respiration in male and female mice.

Physiological reports

McGowan EM,Ehrlicher SE,Stierwalt HD,Robinson MM,Newsom SA

PA5-72948 was used in Western Blotting to determine physiologic adaptations in mitochondrial respiratory capacity in skeletal muscle during short-term consumption of western diet (WD), including if adaptive responses to WD-feeding are modified by concurrent exercise training or may be sex-specific.

Thu Dec 01 00:00:00 EST 2022

Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake.

Nature metabolism

Habtemichael EN,Li DT,Camporez JP,Westergaard XO,Sales CI,Liu X,López-Giráldez F,DeVries SG,Li H,Ruiz DM,Wang KY,Sayal BS,González Zapata S,Dann P,Brown SN,Hirabara S,Vatner DF,Goedeke L,Philbrick W,Shulman GI,Bogan JS

PA5-72948 was used in Immunoprecipitation to show that this TUG cleavage pathway regulates both insulin-stimulated glucose uptake in muscle and organism-level energy expenditure.

Mon Mar 01 00:00:00 EST 2021

Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake.

Nature metabolism

PA5-72948 was used in Immunoprecipitation to show that this TUG cleavage pathway regulates both insulin-stimulated glucose uptake in muscle and organism-level energy expenditure.

Mon Mar 01 00:00:00 EST 2021

Insulin-stimulated endoproteolytic TUG cleavage links energy expenditure with glucose uptake.

Nature metabolism

PA5-72948 was used in Immunoprecipitation to show that this TUG cleavage pathway regulates both insulin-stimulated glucose uptake in muscle and organism-level energy expenditure.

Mon Mar 01 00:00:00 EST 2021

A novel oral nutritional supplement improves gait speed and mitochondrial functioning compared to standard care in older adults with (or at risk of) undernutrition: results from a randomized controlled trial.

Aging

Grootswagers P,Smeets E,Oteng AB,Groot L

PA5-72948 was used in Western Blot to compare the effects of a novel oral nutritional supplement with a standard supplement on body composition, physical functioning, blood markers, and skeletal muscle gene and protein expression in older adults with undernutrition.

Fri Apr 02 00:00:00 EDT 2021

Identification of transcription factor co-regulators that drive prostate cancer progression.

Scientific reports

Siddappa M,Wani SA,Long MD,Leach DA,Mathé EA,Bevan CL,Campbell MJ

Published figure using PGC1 alpha polyclonal antibody (Product # PA5-72948) in Western Blot

Mon Nov 23 00:00:00 EST 2020

Identification of transcription factor co-regulators that drive prostate cancer progression.

Scientific reports

Siddappa M,Wani SA,Long MD,Leach DA,Mathé EA,Bevan CL,Campbell MJ

Published figure using PGC1 alpha polyclonal antibody (Product # PA5-72948) in Western Blot

Mon Nov 23 00:00:00 EST 2020

Whey protein sweetened with Stevia rebaudiana Bertoni (Bert.) increases mitochondrial biogenesis markers in the skeletal muscle of resistance-trained rats.

Nutrition & metabolism

Lima YC,Kurauti MA,da Fonseca Alves G,Ferezini J,Piovan S,Malta A,de Almeida FLA,Gomes RM,de Freitas Mathias PC,Milani PG,da Costa SC,Mareze-Costa CE

PA5-72948 was used in Western Blotting to study the effects of whey protein sweetened with S. rebaudiana on physical performance and mitochondrial biogenesis markers in skeletal muscle.

Sun Apr 10 00:00:00 EDT 2022

生物信息学

蛋白别名: L-PGC-1alpha; Ligand effect modulator 6; ligand effect modulator-6; peroxisome proliferative activated receptor gamma coactivator 1; peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; pgc-1 alpha; PGC-1-alpha; PGC-1alpha; PPAR gamma coactivator variant form; PPAR-gamma coactivator 1-alpha; PPARgamma coactivator 1alpha; PPARGC-1-alpha

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基因别名: I79_015506; LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1; PPARGC1A

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UniProt ID: (Human) Q9UBK2, (Rat) Q9QYK2

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Entrez Gene ID: (Human) 10891, (Chinese hamster) 100753651, (Rat) 83516

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功能