Invitrogen

PGP9.5 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10个已发表图片

11 篇参考文献

查看其他56个PGP9.5抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

PGP9.5 Antibody in Immunohistochemistry (IHC)

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PGP9.5 Antibody (38-1000) in IHC

Immunohistochemical staining of Alzheimer's disease brain tissue using Rb anti-UCH-L1 (C-term) (Product # 38-1000).

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产品信息

38-1000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看9篇已发表文章 9篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Chicken, Human, Mouse, Pig, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from an internal region of the human USF-1 protein, isoforms 1 and 2, which are identical to mouse, rat, dog, and bovine isoform 1 and to mouse and dog isoform 2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533355

靶标信息

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sensory neuron dysfunction in orthotopic mouse models of colon cancer.

Journal of neuroinflammation

Balogh M,Zhang J,Gaffney CM,Kalakuntla N,Nguyen NT,Trinh RT,Aguilar C,Pham HV,Milutinovic B,Nichols JM,Mahalingam R,Shepherd AJ

38-1000 was used in Immunohistochemistry to demonstrate that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process.

Fri Aug 12 00:00:00 EDT 2022

Sensory neuron dysfunction in orthotopic mouse models of colon cancer.

Journal of neuroinflammation

Balogh M,Zhang J,Gaffney CM,Kalakuntla N,Nguyen NT,Trinh RT,Aguilar C,Pham HV,Milutinovic B,Nichols JM,Mahalingam R,Shepherd AJ

38-1000 was used in Immunohistochemistry to demonstrate that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process.

Fri Aug 12 00:00:00 EDT 2022

Chemical phenotypes of intrinsic cardiac neurons in the newborn pig (Sus scrofa domesticus Erxleben, 1777).

Journal of morphology

Ragauskas T,Rysevaite-Kyguoliene K,Pauziene N,Inokaitis H,Pauza DH

38-1000 was used in Immunohistochemistry to demonstrate that intrinsic cardiac ganglionated plexus is not fully developed in the newborn pig despite of dense network of neuronal processes and numerous signs of neural contacts within ganglia.

Sat Jan 01 00:00:00 EST 2022

NLRP3 Promotes Diabetic Bladder Dysfunction and Changes in Symptom-Specific Bladder Innervation.

Diabetes

Hughes FM,Hirshman NA,Inouye BM,Jin H,Stanton EW,Yun CE,Davis LG,Routh JC,Purves JT

Published figure using PGP9.5 polyclonal antibody (Product # 38-1000) in Immunohistochemistry

Fri Feb 01 00:00:00 EST 2019

Bladder decompensation and reduction in nerve density in a rat model of chronic bladder outlet obstruction are attenuated with the NLRP3 inhibitor glyburide.

American journal of physiology. Renal physiology

Hughes FM,Sexton SJ,Ledig PD,Yun CE,Jin H,Purves JT

38-1000 was used in Immunohistochemistry to suggest a critical role for NLRP3 in mediating bladder decompensation and nerve density during chronic BOO.

Tue Jan 01 00:00:00 EST 2019

Inhibition of UCH-L1 Deubiquitinating Activity with Two Forms of LDN-57444 Has Anti-Invasive Effects in Metastatic Carcinoma Cells.

International journal of molecular sciences

Kobayashi E,Hwang D,Bheda-Malge A,Whitehurst CB,Kabanov AV,Kondo S,Aga M,Yoshizaki T,Pagano JS,Sokolsky M,Shakelford J

Published figure using PGP9.5 polyclonal antibody (Product # 38-1000) in Western Blot

Wed Jul 31 00:00:00 EDT 2019

Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation.

Immunity

Vasamsetti SB,Florentin J,Coppin E,Stiekema LCA,Zheng KH,Nisar MU,Sembrat J,Levinthal DJ,Rojas M,Stroes ESG,Kim K,Dutta P

38-1000 was used in Immunohistochemistry to demonstrate that catecholamines produced by leukocytes and sympathetic nerve termini promote GMP proliferation and myeloid cell development.

Tue Jul 17 00:00:00 EDT 2018

NLRP3/IL-1β mediates denervation during bladder outlet obstruction in rats.

Neurourology and urodynamics

Lütolf R,Hughes FM,Inouye BM,Jin H,McMains JC,Pak ES,Hannan JL,Purves JT

Published figure using PGP9.5 polyclonal antibody (Product # 38-1000) in Immunohistochemistry

Thu Mar 01 00:00:00 EST 2018

NLRP3/IL-1β mediates denervation during bladder outlet obstruction in rats.

Neurourology and urodynamics

Lütolf R,Hughes FM,Inouye BM,Jin H,McMains JC,Pak ES,Hannan JL,Purves JT

Published figure using PGP9.5 polyclonal antibody (Product # 38-1000) in Immunohistochemistry

Thu Mar 01 00:00:00 EST 2018

C-Terminal Farnesylation of UCH-L1 Plays a Role in Transport of Epstein-Barr Virus Primary Oncoprotein LMP1 to Exosomes.

mSphere

Kobayashi E,Aga M,Kondo S,Whitehurst C,Yoshizaki T,Pagano JS,Shackelford J

Published figure using PGP9.5 polyclonal antibody (Product # 38-1000) in Western Blot

Wed Nov 20 00:00:00 EST 2019

USP7 small-molecule inhibitors interfere with ubiquitin binding.

Nature

Kategaya L,Di Lello P,Rougé L,Pastor R,Clark KR,Drummond J,Kleinheinz T,Lin E,Upton JP,Prakash S,Heideker J,McCleland M,Ritorto MS,Alessi DR,Trost M,Bainbridge TW,Kwok MCM,Ma TP,Stiffler Z,Brasher B,Tang Y,Jaishankar P,Hearn BR,Renslo AR,Arkin MR,Cohen F,Yu K,Peale F,Gnad F,Chang MT,Klijn C,Blackwood E,Martin SE,Forrest WF,Ernst JA,Ndubaku C,Wang X,Beresini MH,Tsui V,Schwerdtfeger C,Blake RA,Murray J,Maurer T,Wertz IE

38-1000 was used in ELISA, Immunohistochemistry, Western Blot to investigate the ubiquitin system in eukaryotes, and the role of USP7.

Thu Oct 26 00:00:00 EDT 2017

Specific deletion of Cdh2 in Sertoli cells leads to altered meiotic progression and subfertility of mice.

Biology of reproduction

Jiang X,Ma T,Zhang Y,Zhang H,Yin S,Zheng W,Wang L,Wang Z,Khan M,Sheikh SW,Bukhari I,Iqbal F,Cooke HJ,Shi Q

38-1000 was used in immunohistochemistry to investigate the role of Cdh2 in the meiotic progression of Sertoli cells and fertility of mice

Sun Mar 01 00:00:00 EST 2015

Identification of a novel developmental mechanism in the generation of mesothelia.

Development (Cambridge, England)

Winters NI,Thomason RT,Bader DM

38-1000 was used in immunohistochemistry to study the fate of mesothelial progenitor cells.

Wed Aug 01 00:00:00 EDT 2012

生物信息学

蛋白别名: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; UCH-L1; UCHL

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基因别名: AW822034; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1

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UniProt ID: (Human) P09936, (Mouse) Q9R0P9, (Rat) Q00981

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Entrez Gene ID: (Human) 7345, (Mouse) 22223, (Rat) 29545

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