Proteintech

PIEZO1 (extracellular domain) Polyclonal Antibody

PIEZO1 (extracellular domain) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PIEZO1 (extracellular domain) Antibody (28511-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PIEZO1 (extracellular domain) Antibody in Western Blot (WB)

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产品信息

28511-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:300

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

免疫沉淀 (IP)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

FAM38A Fusion Protein Ag29858

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

300 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

产品详细信息

Aliquoting is unnecessary for -20°C storage.

靶标信息

Piezo1 is a pore-forming subunit of a mechanosensitive non-specific cation channel, and generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Structurally, Piezo1 contains 36 transmembrane domains and functions as a homotetramer. Piezo1 plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, Piezo1 may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Piezo1 acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation and plays a key role in blood vessel formation and vascular structure in both development and adult physiology. The protein encoded by the Piezo1 gene is a mechanically-activated ion channel that links mechanical forces to biological signals. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. Diseases associated with PIEZO1 include Dehydrated Hereditary Stomatocytosis with/without Pseudohyperkalemia And/Or Perinatal Edema and Lymphedema.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease.

Neuron

Hu J,Chen Q,Zhu H,Hou L,Liu W,Yang Q,Shen H,Chai G,Zhang B,Chen S,Cai Z,Wu C,Hong F,Li H,Chen S,Xiao N,Wang ZX,Zhang X,Wang B,Zhang L,Mo W

Wed Jan 04 00:00:00 EST 2023

Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease.

Neuron

Hu J,Chen Q,Zhu H,Hou L,Liu W,Yang Q,Shen H,Chai G,Zhang B,Chen S,Cai Z,Wu C,Hong F,Li H,Chen S,Xiao N,Wang ZX,Zhang X,Wang B,Zhang L,Mo W

Wed Jan 04 00:00:00 EST 2023

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes.

Glia

Wan Y,Wang H,Fan X,Bao J,Wu S,Liu Q,Yan X,Zhang J,Jin ZB,Xiao B,Wang N

Mon May 01 00:00:00 EDT 2023

Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes.

Glia

Wan Y,Wang H,Fan X,Bao J,Wu S,Liu Q,Yan X,Zhang J,Jin ZB,Xiao B,Wang N

Mon May 01 00:00:00 EDT 2023

Mechanosensitive Piezo1 channels mediate renal fibrosis.

JCI insight

Zhao X,Kong Y,Liang B,Xu J,Lin Y,Zhou N,Li J,Jiang B,Cheng J,Li C,Wang W

Fri Apr 08 00:00:00 EDT 2022

The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism.

Heliyon

Ye X,Xia Y,Zheng Y,Chen W,Chen Z,Cheng Z,Wang B

Thu Sep 01 00:00:00 EDT 2022

The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism.

Heliyon

Ye X,Xia Y,Zheng Y,Chen W,Chen Z,Cheng Z,Wang B

Thu Sep 01 00:00:00 EDT 2022

Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Diem K,Fauler M,Fois G,Hellmann A,Winokurow N,Schumacher S,Kranz C,Frick M

Tue Sep 01 00:00:00 EDT 2020

Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Diem K,Fauler M,Fois G,Hellmann A,Winokurow N,Schumacher S,Kranz C,Frick M

Tue Sep 01 00:00:00 EDT 2020

Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Diem K,Fauler M,Fois G,Hellmann A,Winokurow N,Schumacher S,Kranz C,Frick M

Tue Sep 01 00:00:00 EDT 2020

生物信息学

蛋白别名: family with sequence similarity 38, member A; Membrane protein induced by beta-amyloid treatment; Mib; Piezo-type mechanosensitive ion channel component 1; Protein FAM38A

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基因别名: DHS; FAM38A; KIAA0233; LMPH3; Mib; PIEZO1

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UniProt ID: (Human) Q92508

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Entrez Gene ID: (Human) 9780

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功能

cation channel activity mechanically-gated ion channel activity

参与通路

cation transport positive regulation of integrin activation positive regulation of cell-cell adhesion mediated by integrin cation transmembrane transport