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PIK3CA Antibody (MA5-38365) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PIK3CA in HeLa cells using PIK3CA Monoclonal Antibody (Product # MA5-38365) diluted at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
产品信息
MA5-38365
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
10E5
抗原
A synthesized peptide derived from human PI 3 Kinase catalytic subunit alpha
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
内含物
0.02% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2898279
产品详细信息
This antibody has been tested in direct-ELISA
靶标信息
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2.7.1.153; EC:2.7.11.1; GRB1; MGC142161; MGC142163; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalytic, alpha polypeptide; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform; Phosphoinositide 3-kinase alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; phosphoinositide-3-kinase, catalytic, alpha polypeptide; PI3-kinase p110 subunit alpha; PI3-kinase subunit alpha; PI3K alpha; PI3K-alpha; PI3Kalpha; pi3kca; PIK3R1; pk3ca; ptdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA
基因别名: 6330412C24Rik; caPI3K; CLOVE; CWS5; MCAP; MCM; MCMTC; p110; p110-alpha; p110alpha; PI3K; PI3K-alpha; PIK3CA
UniProt ID: (Human) P42336, (Mouse) P42337
Entrez Gene ID: (Human) 5290, (Mouse) 18706
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
1-phosphatidylinositol-3-kinase activity
protein kinase activator activity
phosphatidylinositol 3-kinase activity
1-phosphatidylinositol-4-phosphate 3-kinase activity
insulin receptor substrate binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
nucleotide binding
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
kinase
angiogenesis
liver development
vasculature development
glucose metabolic process
protein phosphorylation
phosphatidylinositol biosynthetic process
epidermal growth factor receptor signaling pathway
regulation of gene expression
regulation of phosphatidylinositol 3-kinase signaling
platelet activation
T cell costimulation
activation of protein kinase activity
positive regulation of peptidyl-serine phosphorylation
phosphatidylinositol-3-phosphate biosynthetic process
insulin receptor signaling pathway via phosphatidylinositol 3-kinase
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
regulation of multicellular organism growth
regulation of cellular respiration
protein kinase B signaling
negative regulation of neuron apoptotic process
endothelial cell migration
hypomethylation of CpG island
phosphatidylinositol phosphorylation
vascular endothelial growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
T cell receptor signaling pathway
leukocyte migration
cardiac muscle contraction
adipose tissue development
cellular response to glucose stimulus
energy homeostasis
mitophagy in response to mitochondrial depolarization
negative regulation of fibroblast apoptotic process
regulation of genetic imprinting
negative regulation of anoikis
adaptive immune response
inflammatory response
phosphatidylinositol 3-kinase signaling
phosphorylation
innate immune response
positive regulation of protein kinase activity
cell chemotaxis
