Invitrogen

PINK1 Polyclonal Antibody

查看其他21个PINK1抗体

抗体检测数据 (2)

PINK1 Antibody in Western Blot (WB)

PINK1 Antibody in Western Blot (WB)

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PINK1 Antibody (PA5-18770) in WB

Western blot analysis of PINK1 using PINK1 Polyclonal Antibody (Product # PA5-18770) (1 µg/mL) in staining of Jurkat (A) and (1.5 µg/mL) HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

PINK1 Antibody in Western Blot (WB)

PINK1 Antibody in Flow Cytometry (Flow)

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PINK1 Antibody in Western Blot (WB) — Western blot analysis of PINK1 using PINK1 Polyclonal Antibody (Product # PA5-18770) (1 µg/mL) in staining of Jurkat (A) and (1.5 µg/mL) HeLa (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

PINK1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of PINK1 using PINK1 Polyclonal Antibody (Product # PA5-18770) in paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

产品信息

PA5-18770

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

-

流式细胞分析 (Flow)

10 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (QGKAHLESRSYQEAQ) corresponding to the internal amino acids of PINK1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10989348

产品详细信息

This antibody is predicted to react with human and mouse based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

靶标信息

PINK1 (PTEN-induced putative kinase 1) protects from mitochondrial dysfunction in cells by conferring different autophosphorylation activity to mitochondrial genes. The kinase domain of PINK1 appears to be critical in its protective effects. Mitochondrial proteins are critical for the regulation of cellular energy and adaptation to stress. Particularly in brain cells, mitochondrial demand is extraordinarily high, in order to accommodate aerobic and anaerobic support of high energy processes. When mitochondrial structure and/or function is disrupted in the brain, neurodegenerative diseases like Parkinson's disease are triggered.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: BRPK; FLJ27236; PINK 1; PINK-1; protein kinase BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial

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基因别名: BRPK; PARK6; PINK1

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UniProt ID: (Human) Q9BXM7

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Entrez Gene ID: (Human) 65018

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magnesium ion binding protease binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding calcium-dependent protein kinase activity kinase activity peptidase activator activity ubiquitin protein ligase binding protein kinase B binding C3HC4-type RING finger domain binding non-receptor serine/threonine protein kinase protein modifying enzyme

参与通路

mitophagy positive regulation of protein phosphorylation regulation of oxidative phosphorylation protein phosphorylation ubiquitin-dependent protein catabolic process response to stress response to oxidative stress mitochondrion organization regulation of hydrogen peroxide metabolic process negative regulation of gene expression regulation of mitochondrion organization positive regulation of peptidase activity macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy phosphorylation protein ubiquitination peptidyl-serine phosphorylation respiratory electron transport chain regulation of protein ubiquitination positive regulation of protein ubiquitination activation of protein kinase B activity positive regulation of synaptic transmission, dopaminergic positive regulation of peptidyl-serine phosphorylation positive regulation of dopamine secretion cellular response to oxidative stress positive regulation of protein dephosphorylation intracellular signal transduction peptidyl-serine autophosphorylation TORC2 signaling positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly negative regulation of neuron apoptotic process positive regulation of translation negative regulation of JNK cascade protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of ubiquitin-protein transferase activity regulation of mitochondrial membrane potential positive regulation of protein kinase B signaling regulation of proteasomal protein catabolic process cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitochondrial fission positive regulation of release of cytochrome c from mitochondria negative regulation of mitochondrial fission cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of synaptic vesicle transport negative regulation of autophagosome assembly positive regulation of mitochondrial electron transport, NADH to ubiquinone regulation of mitophagy negative regulation of mitophagy negative regulation of oxidative stress-induced cell death negative regulation of oxidative stress-induced neuron death regulation of protein targeting to mitochondrion negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide positive regulation of cristae formation positive regulation of protein targeting to mitochondrion positive regulation of free ubiquitin chain polymerization regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process positive regulation of ATP biosynthetic process