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PIST Antibody (PA5-121111) in WB
Western blot analysis of PIST in extracts of various cell lines. Samples were incubated with PIST Polyclonal antibody (Product # PA5-121111) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-121111
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human PIST/PIST/GOPC (NP_0010174081)
偶联物
形式
Liquid
浓度
1.89 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914683
产品详细信息
Positive test controls include: HeLa, Mouse kidney, Mouse liver, Mouse brain, Rat brain. The target is usually found in the following locations: Cell junction, Cell projection, Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Peripheral membrane protein, dendrite, postsynaptic cell membrane, postsynaptic density, synapse, trans-Golgi network membrane.
Immunogen sequence: ARLAAKYLDK ELAGRVQQIQ LLGRDMKGPA HDKLWNQLEA EIHLHRHKTV IRACRGRNDL KRPMQAPPGH DQDSLKKSQG VGPIRKVLLL KEDHEGLGIS ITGGKEHGVP ILISEIHPGQ PADRCGGLHV GDAILAVNGV NLRDTKHKEA VTILSQQRGE IEFEVVYVAP EVDSDDENVE YEDESGHRYR LYLDELEGGG NPGASCKDTS GEIKVLQGFN KKAVTDTHEN GDLGTASETP LDDGASKLDD LHTLYHKKSY
靶标信息
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CFTR-associated ligand; dJ94G16.2 PIST; Fused in glioblastoma; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated PDZ and coiled-coil motif containing protein; Golgi-associated PDZ and coiled-coil motif-containing protein; PDZ domain-containing protein PIST; PDZ protein interacting specifically with TC10; PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10; PIST
基因别名: 2210402P09Rik; AI844555; CAL; dJ94G16.2; FIG; GOPC; GOPC1; PIST
UniProt ID: (Human) Q9HD26, (Mouse) Q8BH60
Entrez Gene ID: (Human) 57120, (Mouse) 94221, (Rat) 309774
