Loading ...

Proteintech

PKM1 Polyclonal Antibody

81 篇参考文献

查看其他2个PKM1抗体

说明书

实验方案

技术支持

货号 15821-1-AP

此产品在中国无法购买

150 µL

说明书

实验方案

技术支持

抗体检测数据 (27)

Application

PKM1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 27

PKM1 Antibody (15821-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

PKM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PKM1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

15821-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:4,000

查看58篇已发表文章 58篇已发表文章

免疫组化 (IHC)

查看11篇已发表文章 11篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:400

免疫细胞化学 (ICC/IF)

1:20-1:200

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

0.5-4.0 µg

查看3篇已发表文章 3篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

不适用 (N/A)

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide

偶联物

Unconjugated

形式

Liquid

浓度

0.45 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

This antibody is specific to PKM1 isoform.

靶标信息

PKM1 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

PKM2-mediated neuronal hyperglycolysis enhances the risk of Parkinson's disease in diabetic rats.

Journal of pharmaceutical analysis

Zhao Y,Wang Y,Wu Y,Tao C,Xu R,Chen Y,Qian L,Xu T,Lian X

Wed Feb 01 00:00:00 UTC 2023

Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway.

Brain sciences

Li X,Zhou R,Peng H,Peng J,Li Q,Mei M

Fri Feb 03 00:00:00 UTC 2023

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence.

Nature communications

Wu Y,Tang L,Huang H,Yu Q,Hu B,Wang G,Ge F,Yin T,Li S,Yu X

Fri Mar 10 00:00:00 UTC 2023

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence.

Nature communications

Wu Y,Tang L,Huang H,Yu Q,Hu B,Wang G,Ge F,Yin T,Li S,Yu X

Fri Mar 10 00:00:00 UTC 2023

Paeoniflorin ameliorates airway inflammation and immune response in ovalbumin induced asthmatic mice: From oxidative stress to autophagy.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Han X,Hu S,Yang Q,Sang X,Tang D,Cao G

Tue Feb 01 00:00:00 UTC 2022

ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.

Cancer research

Ma WK,Voss DM,Scharner J,Costa ASH,Lin KT,Jeon HY,Wilkinson JE,Jackson M,Rigo F,Bennett CF,Krainer AR

Tue Mar 01 00:00:00 UTC 2022

GM3 synthase deficiency increases brain glucose metabolism in mice.

Molecular genetics and metabolism

Bharathi SS,Zhang BB,Paul E,Zhang Y,Schmidt AV,Fowler B,Wu Y,Tiemeyer M,Inamori KI,Inokuchi JI,Goetzman ES

Thu Dec 01 00:00:00 UTC 2022

PT109, a novel multi-kinase inhibitor suppresses glioblastoma multiforme through cell reprogramming: Involvement of PTBP1/PKM1/2 pathway.

European journal of pharmacology

Yang Y,Tu Y,Lu J,Chen Q,Zhu Z,Peng W,Zhu W,Wen S,Zhang J,Yin W,Pi R

Tue Apr 05 00:00:00 UTC 2022

Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

Journal of the American Heart Association

Ni L,Lin B,Hu L,Zhang R,Fu F,Shen M,Yang J,Shi D

Tue Jun 07 00:00:00 UTC 2022

A non-canonical role for pyruvate kinase M2 as a functional modulator of Ca²⁺ signalling through IP₃ receptors.

Biochimica et biophysica acta. Molecular cell research

Lavik AR,McColl KS,Lemos FO,Kerkhofs M,Zhong F,Harr M,Schlatzer D,Hamada K,Mikoshiba K,Crea F,Bultynck G,Bootman MD,Parys JB,Distelhorst CW

Fri Apr 01 00:00:00 UTC 2022

Inhibiting pyruvate kinase muscle isoform 2 regresses group 2 pulmonary hypertension induced by supra-coronary aortic banding.

Acta physiologica (Oxford, England)

Xiong PY,Motamed M,Chen KH,Dasgupta A,Potus F,Tian L,Martin A,Mewburn J,Jones O,Thébaud A,Archer SL

Tue Feb 01 00:00:00 UTC 2022

Inhibiting pyruvate kinase muscle isoform 2 regresses group 2 pulmonary hypertension induced by supra-coronary aortic banding.

Acta physiologica (Oxford, England)

Xiong PY,Motamed M,Chen KH,Dasgupta A,Potus F,Tian L,Martin A,Mewburn J,Jones O,Thébaud A,Archer SL

Tue Feb 01 00:00:00 UTC 2022

Celastrol mitigates inflammation in sepsis by inhibiting the PKM2-dependent Warburg effect.

Military Medical Research

Luo P,Zhang Q,Zhong TY,Chen JY,Zhang JZ,Tian Y,Zheng LH,Yang F,Dai LY,Zou C,Li ZJ,Liu JH,Wang JG

Fri May 20 00:00:00 UTC 2022

Lactylation of PKM2 Suppresses Inflammatory Metabolic Adaptation in Pro-inflammatory Macrophages.

International journal of biological sciences

Wang J,Yang P,Yu T,Gao M,Liu D,Zhang J,Lu C,Chen X,Zhang X,Liu Y

Tue Nov 29 00:00:00 UTC 2022

Kaempferol Can Reverse the 5-Fu Resistance of Colorectal Cancer Cells by Inhibiting PKM2-Mediated Glycolysis.

International journal of molecular sciences

Wu H,Du J,Li C,Li H,Guo H,Li Z

Thu Mar 24 00:00:00 UTC 2022

MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung Cancer.

Non-coding RNA

Miglietta S,Girolimetti G,Marchio L,Sollazzo M,Laprovitera N,Coluccelli S,De Biase D,De Leo A,Santini D,Kurelac I,Iommarini L,Ghelli A,Campana D,Ferracin M,Perrone AM,Gasparre G,Porcelli AM

Sun Sep 25 00:00:00 UTC 2022

Capsaicin ameliorates inflammation in a TRPV1-independent mechanism by inhibiting PKM2-LDHA-mediated Warburg effect in sepsis.

Cell chemical biology

Zhang Q,Luo P,Xia F,Tang H,Chen J,Zhang J,Liu D,Zhu Y,Liu Y,Gu L,Zheng L,Li Z,Yang F,Dai L,Liao F,Xu C,Wang J

Thu Aug 18 00:00:00 UTC 2022

Spatiotemporal feedforward between PKM2 tetramers and mTORC1 prompts mTORC1 activation.

Physical biology

Xia Y,Wang S,Song C,Luo RY

Mon Jun 13 00:00:00 UTC 2022

Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury.

Biomedicines

Sun X,Shi F,Wang W,Wu Y,Qu S,Li J,Liang H,Zen K

Sat May 21 00:00:00 UTC 2022

Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.

Arteriosclerosis, thrombosis, and vascular biology

James J,Zemskova M,Eccles CA,Varghese MV,Niihori M,Barker NK,Luo M,Mandarino LJ,Langlais PR,Rafikova O,Rafikov R

Mon Feb 01 00:00:00 UTC 2021

The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.

Nature communications

Zheng F,Chen J,Zhang X,Wang Z,Chen J,Lin X,Huang H,Fu W,Liang J,Wu W,Li B,Yao H,Hu H,Song E

Fri Feb 26 00:00:00 UTC 2021

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Aging

Han J,Zhao Z,Zhang N,Yang Y,Ma L,Feng L,Zhang X,Zuo J,Fan Z,Wang Y,Song Y,Wang G

Wed Jan 20 00:00:00 UTC 2021

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Aging

Han J,Zhao Z,Zhang N,Yang Y,Ma L,Feng L,Zhang X,Zuo J,Fan Z,Wang Y,Song Y,Wang G

Wed Jan 20 00:00:00 UTC 2021

Dihydropyrimidinase Like 2 Promotes Bladder Cancer Progression via Pyruvate Kinase M2-Induced Aerobic Glycolysis and Epithelial-Mesenchymal Transition.

Frontiers in cell and developmental biology

Zou J,Huang R,Chen Y,Huang X,Li H,Liang P,Chen S

Mon Apr 25 00:00:00 UTC 2022

Kaempferol Reverses Aerobic Glycolysis via miR-339-5p-Mediated PKM Alternative Splicing in Colon Cancer Cells.

Journal of agricultural and food chemistry

Wu H,Cui M,Li C,Li H,Dai Y,Cui K,Li Z

Wed Mar 17 00:00:00 UTC 2021

Downregulation of GLUT3 impairs STYK1/NOK-mediated metabolic reprogramming and proliferation in NIH-3T3 cells.

Oncology letters

Shi W,Fu Y,Wang Y

Thu Jul 01 00:00:00 UTC 2021

Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.

The EMBO journal

Xu Y,Shi T,Cui X,Yan L,Wang Q,Xu X,Zhao Q,Xu X,Tang QQ,Tang H,Pan D

Wed Dec 15 00:00:00 UTC 2021

Differential localization patterns of pyruvate kinase isoforms in murine naïve, formative, and primed pluripotent states.

Experimental cell research

Dierolf JG,Watson AJ,Betts DH

Sun Aug 15 00:00:00 UTC 2021

Differential localization patterns of pyruvate kinase isoforms in murine naïve, formative, and primed pluripotent states.

Experimental cell research

Dierolf JG,Watson AJ,Betts DH

Sun Aug 15 00:00:00 UTC 2021

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

International journal of biological sciences

Chen X,Sun N,Li R,Sang X,Li X,Zhao J,Han J,Yang J,Ikezoe T

Thu Mar 24 00:00:00 UTC 2022

Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension.

American journal of physiology. Lung cellular and molecular physiology

Varghese MV,James J,Rafikova O,Rafikov R

Thu Apr 01 00:00:00 UTC 2021

Deubiquitinase PSMD14 promotes ovarian cancer progression by decreasing enzymatic activity of PKM2.

Molecular oncology

Sun T,Liu Z,Bi F,Yang Q

Wed Dec 01 00:00:00 UTC 2021

Myostatin Mutation Promotes Glycolysis by Increasing Phosphorylation of Phosphofructokinase via Activation of PDE5A-cGMP-PKG in Cattle Heart.

Frontiers in cell and developmental biology

Gu M,Zhou X,Zhu L,Gao Y,Gao L,Bai C,Yang L,Li G

Wed Feb 16 00:00:00 UTC 2022

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

Nature communications

Wang C,Li Y,Yan S,Wang H,Shao X,Xiao M,Yang B,Qin G,Kong R,Chen R,Zhang N

Mon Jun 22 00:00:00 UTC 2020

Mutation Promoted IL-6 Production and Glycolysis via Mediating PKM1 Stabilization in Macrophages.

Frontiers in immunology

Li R,Sun N,Chen X,Li X,Zhao J,Cheng W,Hua H,Fukatsu M,Mori H,Takahashi H,Ohkawara H,Fukami M,Okamoto M,Hamazaki Y,Zheng K,Yang J,Ikezoe T

Thu Jul 15 00:00:00 UTC 2021

A glycolysis-based 4-mRNA signature correlates with the prognosis and cell cycle process in patients with bladder cancer.

Cancer cell international

Zhang C,Gou X,He W,Yang H,Yin H

Thu Apr 14 00:00:00 UTC 2022

Overexpression of Pyruvate Kinase M2 in Tumor Tissues Is Associated with Poor Prognosis in Patients with Hepatocellular Carcinoma.

Pathology oncology research : POR

Zhao R,Li L,Yang J,Niu Q,Wang H,Qin X,Zhu N,Shi A

Wed Apr 01 00:00:00 UTC 2020

Intestinal epithelial PKM2 serves as a safeguard against experimental colitis via activating β-catenin signaling.

Mucosal immunology

Sun X,Yao L,Liang H,Wang D,He Y,Wei Y,Ye L,Wang K,Li L,Chen J,Zhang CY,Xu G,Wang F,Zen K

Fri Nov 01 00:00:00 UTC 2019

Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NAD⁺ Metabolome and Induces Transcriptomic and Anti-inflammatory Signatures.

Cell reports

Elhassan YS,Kluckova K,Fletcher RS,Schmidt MS,Garten A,Doig CL,Cartwright DM,Oakey L,Burley CV,Jenkinson N,Wilson M,Lucas SJE,Akerman I,Seabright A,Lai YC,Tennant DA,Nightingale P,Wallis GA,Manolopoulos KN,Brenner C,Philp A,Lavery GG

Tue Aug 13 00:00:00 UTC 2019

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Nature immunology

Huang D,Chen J,Yang L,Ouyang Q,Li J,Lao L,Zhao J,Liu J,Lu Y,Xing Y,Chen F,Su F,Yao H,Liu Q,Su S,Song E

Mon Oct 01 00:00:00 UTC 2018

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.

Nucleic acids research

Hilander T,Zhou XL,Konovalova S,Zhang FP,Euro L,Chilov D,Poutanen M,Chihade J,Wang ED,Tyynismaa H

Thu Jan 25 00:00:00 UTC 2018

Editing activity for eliminating mischarged tRNAs is essential in mammalian mitochondria.

Nucleic acids research

Hilander T,Zhou XL,Konovalova S,Zhang FP,Euro L,Chilov D,Poutanen M,Chihade J,Wang ED,Tyynismaa H

Thu Jan 25 00:00:00 UTC 2018

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Physiological genomics

Williams AL,Khadka V,Tang M,Avelar A,Schunke KJ,Menor M,Shohet RV

Sun Jul 01 00:00:00 UTC 2018

HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction.

Physiological genomics

Williams AL,Khadka V,Tang M,Avelar A,Schunke KJ,Menor M,Shohet RV

Sun Jul 01 00:00:00 UTC 2018

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

PloS one

Ma B,Chen J,Mu Y,Xue B,Zhao A,Wang D,Chang D,Pan Y,Liu J

Mon Feb 11 00:00:00 UTC 2019

Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.

PloS one

Ma B,Chen J,Mu Y,Xue B,Zhao A,Wang D,Chang D,Pan Y,Liu J

Mon Feb 11 00:00:00 UTC 2019

Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway.

Frontiers in pharmacology

Jiang H,Zhang S,Song T,Guan X,Zhang R,Chen X

Tue Sep 29 00:00:00 UTC 2020

Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway.

Frontiers in pharmacology

Jiang H,Zhang S,Song T,Guan X,Zhang R,Chen X

Tue Sep 29 00:00:00 UTC 2020

Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial Abnormalities.

Frontiers in cardiovascular medicine

Xiong PY,Tian L,Dunham-Snary KJ,Chen KH,Mewburn JD,Neuber-Hess M,Martin A,Dasgupta A,Potus F,Archer SL

Wed Sep 30 00:00:00 UTC 2020

Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells.

Cellular signalling

Horiuchi Y,Nakatsu D,Kano F,Murata M

Sun Oct 01 00:00:00 UTC 2017

Pyruvate kinase M1 interacts with A-Raf and inhibits endoplasmic reticulum stress-induced apoptosis by activating MEK1/ERK pathway in mouse insulinoma cells.

Cellular signalling

Horiuchi Y,Nakatsu D,Kano F,Murata M

Sun Oct 01 00:00:00 UTC 2017

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

Nature communications

Wei Y,Wang D,Jin F,Bian Z,Li L,Liang H,Li M,Shi L,Pan C,Zhu D,Chen X,Hu G,Liu Y,Zhang CY,Zen K

Mon Jan 09 00:00:00 UTC 2017

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

Nature communications

Wei Y,Wang D,Jin F,Bian Z,Li L,Liang H,Li M,Shi L,Pan C,Zhu D,Chen X,Hu G,Liu Y,Zhang CY,Zen K

Mon Jan 09 00:00:00 UTC 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 UTC 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 UTC 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 UTC 2017

RGD-modified oncolytic adenovirus-harboring shPKM2 exhibits a potent cytotoxic effect in pancreatic cancer via autophagy inhibition and apoptosis promotion.

Cell death & disease

Xu Y,Chu L,Yuan S,Yang Y,Yang Y,Xu B,Zhang K,Liu XY,Wang R,Fang L,Chen Z,Liang Z

Thu Jun 01 00:00:00 UTC 2017

Pkm2 can enhance pluripotency in ESCs and promote somatic cell reprogramming to iPSCs.

Oncotarget

Qin S,Yang D,Chen K,Li H,Zhang L,Li Y,Le R,Li X,Gao S,Kang L

Fri Oct 13 00:00:00 UTC 2017

Pkm2 can enhance pluripotency in ESCs and promote somatic cell reprogramming to iPSCs.

Oncotarget

Qin S,Yang D,Chen K,Li H,Zhang L,Li Y,Le R,Li X,Gao S,Kang L

Fri Oct 13 00:00:00 UTC 2017

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression.

Cell death & disease

Wei L,Dai Y,Zhou Y,He Z,Yao J,Zhao L,Guo Q,Yang L

Thu Jul 20 00:00:00 UTC 2017

Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression.

Cell death & disease

Wei L,Dai Y,Zhou Y,He Z,Yao J,Zhao L,Guo Q,Yang L

Thu Jul 20 00:00:00 UTC 2017

PKM2 uses control of HuR localization to regulate p27 and cell cycle progression in human glioblastoma cells.

International journal of cancer

Mukherjee J,Ohba S,See WL,Phillips JJ,Molinaro AM,Pieper RO

Fri Jul 01 00:00:00 UTC 2016

PKM2 uses control of HuR localization to regulate p27 and cell cycle progression in human glioblastoma cells.

International journal of cancer

Mukherjee J,Ohba S,See WL,Phillips JJ,Molinaro AM,Pieper RO

Fri Jul 01 00:00:00 UTC 2016

M-Type Pyruvate Kinase Isoforms and Lactate Dehydrogenase A in the Mammalian Retina: Metabolic Implications.