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Invitrogen
PKM2 Polyclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 10
PKM2 Antibody (PA5-23034) in ICC/IF
Immunofluorescence analysis of PKM2 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PKM2 Polyclonal Antibody (Product # PA5-23034) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A... View More
产品信息
PA5-23034
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide made to the internal region of human PKM2 protein (within residues 350-450).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 30% glycerol
内含物
0.1% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_11151984
产品详细信息
The target sequence has 95% sequence homology with bovine.
靶标信息
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; isozymes M1/M2; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tu; Tumor M2-PK; Tyrosine-protein kinase PKM2
基因别名: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1
UniProt ID: (Human) P14618, (Mouse) P52480, (Rat) P11980
Entrez Gene ID: (Bovine) 512571, (Human) 5315, (Mouse) 18746, (Rat) 25630
magnesium ion binding
pyruvate kinase activity
protein binding
ATP binding
kinase activity
MHC class II protein complex binding
potassium ion binding
ADP binding
poly(A) RNA binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
catalytic activity
transferase activity
metal ion binding
kinase
response to hypoxia
liver development
ATP biosynthetic process
response to nutrient
response to gravity
programmed cell death
response to muscle inactivity
organ regeneration
response to insulin
pyruvate biosynthetic process
skeletal muscle tissue regeneration
canonical glycolysis
cell-cell adhesion
glucose metabolic process
glycolytic process
metabolic process
phosphorylation
response to organic substance
