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PKN1 Antibody (PA5-87454) in WB
Western blot analysis of PKN1 in extracts of various cell lines. Samples were incubated with PKN1 Polyclonal antibody (Product # PA5-87454) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
产品信息
PA5-87454
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PKN1 (NP_0027323)
偶联物
形式
Liquid
浓度
0.53 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2804162
产品详细信息
Immunogen sequence: MASDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLRREIRKEL KLKEGAENLR RATTDLGRSL GPVELLLRGS SRRLDLLHQQ LQELHAHVVL PDPAATHDGP QSPGAGGPTC SATNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSTKDR KLLLTAQQML QDSKTKIDII RMQLRRALQA GQLENQAAPD DTQGSPDLGA VELRIEELRH HFRVEHAVAE GAKNVLRLLS AAKAPDRKAV SEAQEKLTES NQKLGLLREA LERRLGELPA DHPKGRLLRE ELAAASSAAF; Positive Samples: U-251MG, A-549, 293T, HeLa, HepG2, HT-1080, Mouse kidney, Mouse spleen; Cellular Location: Cell membrane, Cleavage furrow, Cytoplasm, Endosome, Midbody, Nucleus, Peripheral membrane protein
靶标信息
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: PAK-1; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; protein kinase C-related kinase 1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine kinase 3; serine/threonine protein kinase N; Serine/threonine-protein kinase N1
基因别名: DBK; F730027O18Rik; PAK-1; PAK1; PKN; PKN-ALPHA; PKN1; PRK1; PRKCL1; Stk3
UniProt ID: (Human) Q16512, (Mouse) P70268
Entrez Gene ID: (Human) 5585, (Mouse) 320795
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein kinase C binding
protein binding
ATP binding
GTP-Rho binding
ligand-dependent nuclear receptor transcription coactivator activity
histone kinase activity (H3-T11 specific)
histone binding
histone deacetylase binding
Rac GTPase binding
androgen receptor binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
B cell homeostasis
B cell apoptotic process
regulation of germinal center formation
regulation of immunoglobulin production
renal system process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
signal transduction
activation of JUN kinase activity
epithelial cell migration
peptidyl-serine phosphorylation
negative regulation of B cell proliferation
histone H3-T11 phosphorylation
spleen development
regulation of cell motility
negative regulation of protein phosphorylation
regulation of transcription, DNA-templated
phosphorylation
chromatin modification
