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PLA2G5 Antibody (MA1-830) in ICC/IF
Immunofluorescence analysis of Group V secretory phospholipase A2 (PLAG5) was performed using 90% confluent log phase NCCIT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLA2G5 (3G1) Mouse Monoclonal Antibody (Product # MA1-830) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa ... View More
产品信息
MA1-830
产品规格
种属反应
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3G1
抗原
Full length W79A human group V phospholipase A2.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2164744
产品详细信息
MA1-830 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA1-830 detects Group V Secretory Phospholipase A2 from human and mouse samples.
MA1-830 has been successfully used in Western blot procedures. By Western blot, MA1-830 detects a ~15 kDa band representing Group V Secretory Phospholipase A2 from mouse macrophages.
The MA1-830 immunogen is full length W79A human group V phospholipase A2.
靶标信息
This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Ca2+-dependent phospholipase A2; calcium-dependent phospholipase A2; DKFZp686C2294; group V phospholipase A2; MGC46205; Phosphatidylcholine 2-acylhydrolase 5; Phospholipase A2 group V; phospholipase A2, group V; PLA2-10
基因别名: FRFB; GV-PLA2; hVPLA(2); PLA2; PLA2-10; PLA2G5; sPLA2
UniProt ID: (Human) P39877, (Mouse) P97391
Entrez Gene ID: (Human) 5322, (Mouse) 18784
phospholipid metabolic process
phosphatidic acid biosynthetic process
platelet activating factor biosynthetic process
positive regulation of phospholipase activity
lipid catabolic process
leukotriene biosynthetic process
response to cytokine
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
arachidonic acid secretion
response to cAMP
positive regulation of ERK1 and ERK2 cascade
lipid metabolic process
