Invitrogen

PLS3 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

8 篇参考文献

查看其他10个PLS3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

PLS3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

PLS3 Antibody (PA5-27883) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of PLS3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PLS3 Polyclonal Antibody (Product # PA5-27883) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

PLS3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PLS3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PLS3 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with T-Plastin shRNA and decrease in signal intensity was observed in western blot application using T-Plastin antibody (Product # PA5-27883). {KD}

产品信息

PA5-27883

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 66 and 371 of Human T-Plastin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.23 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2545359

产品详细信息

Recommended positive controls: 293T, A431, JurKat, Raji, NIH-3T3, JC, BCL-1.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (91%), Chicken (95%), Rhesus Monkey (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Phospholipid scramblases (PLS) translocate phospholipids bidirectionally between inner and outer leaflets of the plasma membrane and are implicated in apoptosis (1,2). Members of this family, PLS1 and PLS3 are both substrates of Protein kinase C (PKC) delta and are phosphorylated by PKC-delta during apoptosis. PKC-delta translocates to mitochondria during apoptosis and phosphorylates PLS3. Overexpression of PLS3 in the HEK293 cells enhanced apoptosis induced by UV-irradiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Nature communications

Morandell J,Schwarz LA,Basilico B,Tasciyan S,Dimchev G,Nicolas A,Sommer C,Kreuzinger C,Dotter CP,Knaus LS,Dobler Z,Cacci E,Schur FKM,Danzl JG,Novarino G

PA5-27883 was used in Western Blot to provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs.

Mon May 24 00:00:00 EDT 2021

T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.

Nature communications

Garbett D,Bisaria A,Yang C,McCarthy DG,Hayer A,Moerner WE,Svitkina TM,Meyer T

PA5-27883 was used in Western Blot, Immunocytochemistry to identify T-Plastin which acts as an actin stabilizer that promotes membrane protrusions and enables bridging of extracellular matrix(ECM) gaps.

Wed Sep 23 00:00:00 EDT 2020

T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.

Nature communications

Garbett D,Bisaria A,Yang C,McCarthy DG,Hayer A,Moerner WE,Svitkina TM,Meyer T

Wed Sep 23 00:00:00 EDT 2020

T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.

Nature communications

Garbett D,Bisaria A,Yang C,McCarthy DG,Hayer A,Moerner WE,Svitkina TM,Meyer T

Wed Sep 23 00:00:00 EDT 2020

Evaluation of the role of an antioxidant gene in NSC-34 motor neuron-like cells as a model of a motor neuron disease.

Folia morphologica

Alrafiah AR

PA5-27883 was used in Western Blotting to determine the mechanisms underlying the loss of motor neurons.

Mon Jan 25 00:00:00 EST 2021

The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia.

Cancers

Velthaus A,Cornils K,Hennigs JK,Grüb S,Stamm H,Wicklein D,Bokemeyer C,Heuser M,Windhorst S,Fiedler W,Wellbrock J

PA5-27883 was used in Immunocytochemistry-immunoflourescence to identify high expression of actin-binding protein Plastin-3 in the leukemic bone marrow niche contributes to the progression of acute myeloid leukemia.

Sat Oct 26 00:00:00 EDT 2019

Hypoxia-stimulated membrane trafficking requires T-plastin.

Acta physiologica (Oxford, England)

Wottawa M,Naas S,Böttger J,van Belle GJ,Möbius W,Revelo NH,Heidenreich D,von Ahlen M,Zieseniss A,Kröhnert K,Lutz S,Lenz C,Urlaub H,Rizzoli SO,Katschinski DM

PA5-27883 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that T-plastin mediates the hypoxia-induced membrane trafficking, which was associated with an increased actin density in the cells as determined by electron microscopy.

Fri Sep 01 00:00:00 EDT 2017

Circulating tumour cell-derived plastin3 is a novel marker for predicting long-term prognosis in patients with breast cancer.

British journal of cancer

Ueo H,Sugimachi K,Gorges TM,Bartkowiak K,Yokobori T,Müller V,Shinden Y,Ueda M,Ueo H,Mori M,Kuwano H,Maehara Y,Ohno S,Pantel K,Mimori K

PA5-27883 was used in immunocytochemistry to assess the use of epithelial-mesenchymal transition-related biomarker plastin3 (PLS3) as a prognostic factor for breast cancer